Immunofluorescent analysis of PARP in HT-29 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a PARP monoclonal antibody (Product # MA3-950) at a dilution of 1:200 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503). PARP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.
|Tested species reactivity||Bovine, Hamster, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified calf thymus poly (ADP-ribose) polymerase.|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunocytochemistry (ICC)||1:100 - 1:1000|
|Immunofluorescence (IF)||1:100 - 1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:1000 - 1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 7 publications below|
This antibody does not detect PARP from chicken tissues.
By Western blot, this antibody recognizes a 116 kDa protein which corresponds to PARP and the 85 kDa apoptosis-induced cleavage product of prICE and CPP32 from rat thymus extract.
This antibody recognizes an epitope found between amino acids 216-375 in the DNA-binding domain of PARP.
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
New insights into PARP inhibitors' effect on cell cycle and homology-directed DNA damage repair.
MA3-950 was used in western blot to study the off-target effects of the PARP inhibitors olaparib and veliparib on cell cycle progression and homology-directed DNA damage repair
|Jelinic P,Levine DA||Molecular cancer therapeutics (13:1645)||2014|
Identification of TSG101 functional domains and p21 loci required for TSG101-mediated p21 gene regulation.
MA3-950 was used in western blot to study the mechanisms underlying the regulation of p21 gene expression by TSG101
|Lin YS,Chen YJ,Cohen SN,Cheng TH||PloS one (8:null)||2013|
|Not Applicable||Not Cited||
ADP ribosylation by PARP-1 suppresses HOXB7 transcriptional activity.
MA3-950 was used in western blot to investigate the interaction between HOXB7 and poly (ADP-ribose) polymerase-1
|Wu X,Ellmann S,Rubin E,Gil M,Jin K,Han L,Chen H,Kwon EM,Guo J,Ha HC,Sukumar S||PloS one (7:null)||2012|
Cellular dynamics of the negative transcription elongation factor NELF.
MA3-950 was used in western blot to investigate the cellular localization and function of negative transcription elongation factor
|Yung TM,Narita T,Komori T,Yamaguchi Y,Handa H||Experimental cell research (315:1693)||2009|
Role of human transcription elongation factor DSIF in the suppression of senescence and apoptosis.
MA3-950 was used in western blot to investigate the role of human transcription elongation factor DSIF in the suppression of senescence and apoptosis
|Komori T,Inukai N,Yamada T,Yamaguchi Y,Handa H||Genes to cells : devoted to molecular and cellular mechanisms (14:343)||2009|
Apoptosis-inducing factor is a major contributor to neuronal loss induced by neonatal cerebral hypoxia-ischemia.
MA3-950 was used in western blot to characterize defects in harlequin mutant mice
|Zhu C,Wang X,Huang Z,Qiu L,Xu F,Vahsen N,Nilsson M,Eriksson PS,Hagberg H,Culmsee C,Plesnila N,Kroemer G,Blomgren K||Cell death and differentiation (14:775)||2007|
|Not Applicable||Not Cited||
Mechanisms of oncogenic KIT signal transduction in primary gastrointestinal stromal tumors (GISTs).
MA3-950 was used in western blot to identify pathways that are constitutively activated in a KIT-dependent manner in gastrointestinal stromal tumors
|Duensing A,Medeiros F,McConarty B,Joseph NE,Panigrahy D,Singer S,Fletcher CD,Demetri GD,Fletcher JA||Oncogene (23:3999)||2004|