|Tested species reactivity||Bovine, Human, Mouse, Rat|
|Published species reactivity||Rat, Human, Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized peptide corresponding to the N-terminus of cleavage site (214/215) of human PARP.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Human||Not Cited||Disordered IL-33/ST2 activation in decidualizing stromal cells prolongs uterine receptivity in women with recurrent pregnancy loss.||Salker MS,Nautiyal J,Steel JH,Webster Z,Su¿urovi¿ S,Nicou M,Singh Y,Lucas ES,Murakami K,Chan YW,James S,Abdallah Y,Christian M,Croy BA,Mulac-Jericevic B,Quenby S,Brosens JJ||PloS one (7:null)||2013|
A gatekeeper residue for NEDD8-activating enzyme inhibition by MLN4924.
44-698G was used in western blot to elucidate resistance to MLN4924.
|Toth JI,Yang L,Dahl R,Petroski MD||Cell reports (1:309)||2012|
|Not Applicable||Not Cited||
The Bax carboxy-terminal hydrophobic helix does not determine organelle-specific targeting but is essential for maintaining Bax in an inactive state and for stable mitochondrial membrane insertion.
44-698G was used in western blot to investigate the function of the hydrophobic carboxy-terminal tail of Bax
|Brock SE,Li C,Wattenberg BW||Apoptosis : an international journal on programmed cell death (15:14)||2010|
|Human||Not Cited||Genistein inhibits radiation-induced activation of NF-kappaB in prostate cancer cells promoting apoptosis and G2/M cell cycle arrest.||Raffoul JJ,Wang Y,Kucuk O,Forman JD,Sarkar FH,Hillman GG||BMC cancer (6:null)||2006|
|Human||Not Cited||CHIR-258: a potent inhibitor of FLT3 kinase in experimental tumor xenograft models of human acute myelogenous leukemia.||Lopes de Menezes DE,Peng J,Garrett EN,Louie SG,Lee SH,Wiesmann M,Tang Y,Shephard L,Goldbeck C,Oei Y,Ye H,Aukerman SL,Heise C||Clinical cancer research : an official journal of the American Association for Cancer Research (11:5281)||2005|
|Human||Not Cited||Development of immunomodulatory six base-length non-CpG motif oligonucleotides for cancer vaccination.||Filion MC,Filion B,Roy J,Ménard S,Reader S,Phillips NC||Vaccine (22:2480)||2004|
|Human||Not Cited||Calpain 3 is important for muscle regeneration: evidence from patients with limb girdle muscular dystrophies.||Hauerslev S,Sveen ML,Duno M,Angelini C,Vissing J,Krag TO||BMC musculoskeletal disorders (13:null)||2012|
|Mouse||Not Cited||Depletion of alveolar macrophages exerts protective effects in pulmonary tuberculosis in mice.||Leemans JC,Juffermans NP,Florquin S,van Rooijen N,Vervoordeldonk MJ,Verbon A,van Deventer SJ,van der Poll T||Journal of immunology (Baltimore, Md. : 1950) (166:4604)||2001|
Expression of a naturally occurring angiotensin AT(1) receptor cleavage fragment elicits caspase-activation and apoptosis.
44-698G was used in immunocytochemistry to identify the cleavage sites of angiotensin type-1 receptor.
|Cook JL,Singh A,DeHaro D,Alam J,Re RN||American journal of physiology. Cell physiology (301:C1175)||2011|
BRCA1-IRIS overexpression abrogates UV-induced p38MAPK/p53 and promotes proliferation of damaged cells.
44-698G was used in western blot to study the regulation of the p38MAPK-p53-WIP1 pathway by BRCA1-IRIS in stressed and cancer cells
|Chock K,Allison JM,Elshamy WM||Oncogene (29:5274)||2010|
|Human||Not Cited||Skin cells and tissue are capable of using L-ergothioneine as an integral component of their antioxidant defense system.||Markova NG,Karaman-Jurukovska N,Dong KK,Damaghi N,Smiles KA,Yarosh DB||Free radical biology and medicine (46:1168)||2009|
||Genistein inhibits radiation-induced activation of NF-kappaB in prostate cancer cells promoting apoptosis and G2/M cell cycle arrest.||Raffoul JJ,Wang Y,Kucuk O,Forman JD,Sarkar FH,Hillman GG||BMC cancer (6:null)||2006|
||Development of immunomodulatory six base-length non-CpG motif oligonucleotides for cancer vaccination.||Filion MC,Filion B,Roy J,Ménard S,Reader S,Phillips NC||Vaccine (22:2480)||2004|
|Human||Not Cited||Induction of apoptosis in human lung cancer cells by curcumin.||Radhakrishna Pillai G,Srivastava AS,Hassanein TI,Chauhan DP,Carrier E||Cancer letters (208:163)||2004|
|Human||Not Cited||RNA interference is a functional pathway with therapeutic potential in human myeloid leukemia cell lines.||Cioca DP,Aoki Y,Kiyosawa K||Cancer gene therapy (10:125)||2003|
|Human||Not Cited||Peroxisome proliferator-activated receptor gamma ligand troglitazone induces cell cycle arrest and apoptosis of hepatocellular carcinoma cell lines.||Yoshizawa K,Cioca DP,Kawa S,Tanaka E,Kiyosawa K||Cancer (95:2243)||2002|
|Human||Not Cited||Proliferation marker pKi-67 affects the cell cycle in a self-regulated manner.||Schmidt MH,Broll R,Bruch HP,Duchrow M||Journal of cellular biochemistry (87:334)||2002|
|Human||Not Cited||Induction of apoptosis and CD10/neutral endopeptidase expression by jaspamide in HL-60 line cells.||Cioca DP,Kitano K||Cellular and molecular life sciences : CMLS (59:1377)||2002|