|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from C-terminus of human Pax-5 protein|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
PAX-5 is a B cell specificactivator protein (BSAP). In the early stages of B cell developmemt, PAX-5 influences the expression of several B-cell-specific genes, such as CD19 and CD20. PAX-5 is expressed primarily inpro-, pre-, and mature B cells, but not in plasma cells. Interestingly, PAX-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis. Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis. This indicates that PAX-5 is important in B cell development, but may also have a role in proper neuronal development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Increased utilization, verification, and clinical implications of immunocytochemistry: Experience in a northern New England hospital.
MA5-16389 was used in immunohistochemistry to study a northern New England hospital and increased utilization, verification, and clinical implications of immunocytochemistry
|Sauter JL,Ambaye AB,Mount SL||Diagnostic cytopathology (43:688)||2015|