Abnova

PAX2 Monoclonal Antibody (2E4)

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PAX2 Antibody in Western Blot (WB) — PAX2 monoclonal antibody (M02), clone 2E4 Western Blot analysis of PAX2 expression in PC-12 (Cat # L012V1).

PAX2 Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged PAX2 is 0.03 ng/mL as a capture antibody.

Product Details

H00005076-M02

Applications	Tested Dilution	Publications
Western Blot (WB)	1-5 µg/mL	-
ELISA (ELISA)	0.03 ng/mL	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	2E4
Immunogen	PAX2 (NP_000269, 194 a.a. approximately 303 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Conjugate	Unconjugated
Form	Liquid
Concentration	See Label
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: PRSNGEKRKR DEDVSEGSVP NGDSQSGVDS LRKHLRADTF TQQQLEALDR VFERPSYPDV FQASEHIKSE QGNEYSLPAL TPGLDEVKSS LSASTNPELG SNVSGTQTYP

Target Information

The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms' tumors. Persistent expression of Pax-2 in Wilms' tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms' tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: paired box gene 2; paired box homeotic gene 2; Paired box protein Pax-2

Gene Aliases: FSGS7; PAPRS; PAX2

UniProt ID: (Human) Q02962

Entrez Gene ID: (Human) 5076, (Rat) 293992

urogenital system development branching involved in ureteric bud morphogenesis cell fate determination mesonephros development neural tube closure optic cup morphogenesis involved in camera-type eye development mesenchymal to epithelial transition involved in metanephros morphogenesis retinal pigment epithelium development transcription, DNA-templated transcription from RNA polymerase II promoter axonogenesis mesodermal cell fate specification aging visual perception glial cell differentiation optic nerve development optic nerve morphogenesis optic nerve structural organization vestibulocochlear nerve formation response to nutrient levels regulation of metanephros size ureter maturation pronephric field specification inner ear morphogenesis camera-type eye development negative regulation of apoptotic process negative regulation of programmed cell death negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of cytolysis positive regulation of transcription from RNA polymerase II promoter pronephros development brain morphogenesis stem cell differentiation positive regulation of epithelial cell proliferation oxidation-reduction process mesenchymal to epithelial transition optic chiasma development cellular response to hydrogen peroxide cellular response to retinoic acid cellular response to glucose stimulus cellular response to epidermal growth factor stimulus metanephric mesenchyme development positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis metanephric mesenchymal cell differentiation nephric duct formation ureter development metanephric collecting duct development metanephric epithelium development metanephric distal convoluted tubule development metanephric nephron tubule formation positive regulation of metanephric glomerulus development negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis regulation of metanephric nephron tubule epithelial cell differentiation reactive oxygen species metabolic process cochlea development cochlea morphogenesis positive regulation of branching involved in ureteric bud morphogenesis negative regulation of mesenchymal cell apoptotic process involved in metanephros development negative regulation of apoptotic process involved in metanephric collecting duct development negative regulation of apoptotic process involved in metanephric nephron tubule development negative regulation of reactive oxygen species metabolic process positive regulation of metanephric DCT cell differentiation positive regulation of optic nerve formation negative regulation of transcription from RNA polymerase II promoter metanephros development ureteric bud development kidney development positive regulation of cell proliferation pancreas development regulation of apoptotic process regulation of programmed cell death animal organ development paramesonephric duct development cellular response to mechanical stimulus renal system development mesonephric tubule development mesonephric tubule formation mesonephric duct development ureter morphogenesis

Process(es)

core promoter proximal region sequence-specific DNA binding DNA binding protein binding transcription factor binding superoxide-generating NADPH oxidase activity transcription regulatory region DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding C2H2 zinc finger domain binding