Invitrogen

PAX3 Monoclonal Antibody (GT2411)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

View all (25) PAX3 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (4)
Advanced Verification (1)

FIGURE: 1 / 5

PAX3 Antibody (MA5-31582) in WB

Western blot was performed using Anti-PAX3 Monoclonal Antibody, (Product # MA5-31582) and a ~52 kDa band corresponding to PAX3 was observed across the panel tested except T-47D and MCF-7 which are reported to be negative. An uncharacterized band (*) at ~110kDa was also observed across the panel tested. Modified Whole cell extracts (1% SDS) (30 µg lysate) of A-375 (Lane 1), U-87 MG (Lane 2), HEK-293 (Lane 3), T-47D (Lane 4), MCF-7 (Lane 5), Neuro-2a (Lane 6), PC-12 (Lane 7) and NIH/3T3 (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12... View More

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PAX3 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in PAX3 KO cell line compared to wild type control cell line using PAX3 Monoclonal Antibody (Product # MA5-31582). {KO}

Product Details

MA5-31582

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

GT2411

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Human PAX3.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2787209

Product Specific Information

Keep as concentrated solution.

Positive Control: U87-MG, U87-MG nuclear extract.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Pax 3 (Paired Box 3) is a transcription factor that is involved in development of the peripheral nervous system, melanocytes, some vascular smooth muscle, and a number of other derivatives. It regulates neurogenesis in pre-migratory neural crest cells from the dorsal neural tube, and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Alternative splicing results in isoforms having different C-termini.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: homeodomain protein PAX3; HuP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; paired box gene 3; paired box homeotic gene 3; Paired box protein Pax-3; paired domain gene 3; paired domain gene HuP2; PAX3/FKHR fusion

Gene Aliases: CDHS; HUP2; Pax-3; PAX3; Sp; splotch; WS1; WS3

UniProt ID: (Human) P23760, (Mouse) P24610

Entrez Gene ID: (Human) 5077, (Rat) 114502, (Mouse) 18505

Molecular Function: homeodomain transcription factor