Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 2 publications 2 publications |
Immunohistochemistry (IHC) |
- | View 2 publications 2 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:200 | - |
Immunohistochemistry (Frozen) (IHC (F)) |
Assay-dependent | - |
Immunocytochemistry (ICC/IF) |
1:50-1:200 | View 1 publication 1 publication |
Flow Cytometry (Flow) |
1:100 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Human, Mouse |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
13B10-1A10 |
Immunogen |
Purified internal fragment of human recombinant PAX6 expressed in E. coli. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS with 1mg/mL BSA, 30% glycerol |
Contains |
0.05% sodium azide |
Storage conditions |
-20°C |
RRID |
AB_2536820 |
Western blot analysis of MA1-109 detects an ~47 kDa protein in whole cell lysates from early passage human B3 lens epithelial cells and in mouse eye lysate. An additional unknown band at ~36 kDa is also detected in B3 cell lysates. This antibody will detect additional nonspecific bands in lysates with very low basal PAX6 expression. Specificity of the antibody was confirmed in B3 and mouse eye lysates (positive controls) compared to U-2 OS human osteosarcoma and NIH/3T3 mouse embryonic fibroblasts (negative controls).
MA1-109- can be used for immunofluoresence and flow cytometry analysis of Pax6 in human neural stem cells.
This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Aniridia; Aniridia type II protein; Dickie's small eye; Keratitis; MGC17209; Oculorhombin; paired box gene 6; Paired Box Gene-6; paired box homeotic gene-6; Paired box protein Pax-6; small eye
Gene Aliases: 1500038E17Rik; AEY11; AN; AN2; D11S812E; Dey; FVH1; Gsfaey11; MGDA; Pax-6; PAX6; Sey; WAGR
UniProt ID: (Human) P26367, (Mouse) P63015
Entrez Gene ID: (Human) 5080, (Mouse) 18508
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