Invitrogen

PAX6 Monoclonal Antibody (13B10-1A10)

PAX6 Antibody in Immunocytochemistry (ICC/IF)

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PAX6 Antibody (MA1-109) in ICC/IF

Immunofluorescent analysis of PAX6 (green) in human B3 lens epithelial cells (left panel) and negative control U-2 OS cells (right panel). The cells were fixed with formaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 1% Blocker BSA in PBS (Product # 37525) for 15 minutes, all at room temperature. Cells were stained with a PAX6 monoclonal antibody (Product # MA1-109) in 1% Blocker BSA in PBS (Product # 37525) at a dilution of 1:100 for 1 hour at room temperature, and then incubated with ... View More

PAX6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PAX6 Antibody in Immunohistochemistry (Frozen) (IHC (F))

PAX6 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

PAX6 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PAX6 Antibody — The specificity of anti-PAX6 mouse monoclonal antibody (Product # MA1-109) was demonstrated by the immunofluorescence detection of Pax6 expression in positive human B3 lens epithelial cells (left panel) compared to negative control U-2OS cells (right panel). {RE}

Product Details

MA1-109

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

Immunocytochemistry (ICC/IF)

1:50-1:200

View 3 publications 3 publications

Flow Cytometry (Flow)

1:100

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

13B10-1A10

Immunogen

Purified internal fragment of human recombinant PAX6 expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2536820

Product Specific Information

Western blot analysis of MA1-109 detects an ~47 kDa protein in whole cell lysates from early passage human B3 lens epithelial cells and in mouse eye lysate. An additional unknown band at ~36 kDa is also detected in B3 cell lysates. This antibody will detect additional nonspecific bands in lysates with very low basal PAX6 expression. Specificity of the antibody was confirmed in B3 and mouse eye lysates (positive controls) compared to U-2 OS human osteosarcoma and NIH/3T3 mouse embryonic fibroblasts (negative controls).

MA1-109- can be used for immunofluoresence and flow cytometry analysis of Pax6 in human neural stem cells.

Target Information

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An increase in ER stress and unfolded protein response in iPSCs-derived neuronal cells from neuronopathic Gaucher disease patients.

Scientific reports

Pornsukjantra T,Saikachain N,Sutjarit N,Khongkrapan A,Tubsuwan A,Bhukhai K,Tim-Aroon T,Anurathapan U,Hongeng S,Asavapanumas N

Published figure using PAX6 monoclonal antibody (Product # MA1-109) in Flow Cytometry

Mon Apr 22 00:00:00 UTC 2024

miR-137 and miR-122, two outer subventricular zone non-coding RNAs, regulate basal progenitor expansion and neuronal differentiation.

Cell reports

Tomasello U,Klingler E,Niquille M,Mule N,Santinha AJ,de Vevey L,Prados J,Platt RJ,Borrell V,Jabaudon D,Dayer A

MA1-109 was used in Immunohistochemistry to support a cell-type-specific role of miRNA-mediated gene expression in cortical expansion.

Tue Feb 15 00:00:00 UTC 2022

miR-137 and miR-122, two outer subventricular zone non-coding RNAs, regulate basal progenitor expansion and neuronal differentiation.

Cell reports

Tomasello U,Klingler E,Niquille M,Mule N,Santinha AJ,de Vevey L,Prados J,Platt RJ,Borrell V,Jabaudon D,Dayer A

MA1-109 was used in Immunohistochemistry to support a cell-type-specific role of miRNA-mediated gene expression in cortical expansion.

Tue Feb 15 00:00:00 UTC 2022

The Efficiency of Direct Maturation: the Comparison of Two hiPSC Differentiation Approaches into Motor Neurons.

Stem cells international

Schaefers C,Rothmiller S,Thiermann H,Rein T,Schmidt A

Published figure using PAX6 monoclonal antibody (Product # MA1-109) in Western Blot

Thu Dec 22 00:00:00 UTC 2022

3D Interfacial and Spatiotemporal Regulation of Human Neuroepithelial Organoids.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Tang C,Wang X,D'Urso M,van der Putten C,Kurniawan NA

Published figure using PAX6 monoclonal antibody (Product # MA1-109) in Immunocytochemistry

Mon Aug 01 00:00:00 UTC 2022

3D Interfacial and Spatiotemporal Regulation of Human Neuroepithelial Organoids.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Tang C,Wang X,D'Urso M,van der Putten C,Kurniawan NA

Published figure using PAX6 monoclonal antibody (Product # MA1-109) in Immunocytochemistry

Mon Aug 01 00:00:00 UTC 2022

Centrosome defects cause microcephaly by activating the 53BP1-USP28-TP53 mitotic surveillance pathway.

The EMBO journal

Phan TP,Maryniak AL,Boatwright CA,Lee J,Atkins A,Tijhuis A,Spierings DC,Bazzi H,Foijer F,Jordan PW,Stracker TH,Holland AJ

MA1-109 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot to show that the depletion of centrosome proteins in NPCs prolongs mitosis and increases TP53-mediated apoptosis.

Mon Jan 04 00:00:00 UTC 2021

Glycoproteomic analysis of the changes in protein N-glycosylation during neuronal differentiation in human-induced pluripotent stem cells and derived neuronal cells.

Scientific reports

Kimura K,Koizumi T,Urasawa T,Ohta Y,Takakura D,Kawasaki N

Published figure using PAX6 monoclonal antibody (Product # MA1-109) in Immunocytochemistry

Thu May 27 00:00:00 UTC 2021

Glycoproteomic analysis of the changes in protein N-glycosylation during neuronal differentiation in human-induced pluripotent stem cells and derived neuronal cells.

Scientific reports

Kimura K,Koizumi T,Urasawa T,Ohta Y,Takakura D,Kawasaki N

Published figure using PAX6 monoclonal antibody (Product # MA1-109) in Immunocytochemistry

Thu May 27 00:00:00 UTC 2021

Modelling Toxoplasma gondii infection in human cerebral organoids.

Emerging microbes & infections

Seo HH,Han HW,Lee SE,Hong SH,Cho SH,Kim SC,Koo SK,Kim JH

MA1-109 was used in Immunocytochemistry to show that tachyzoites can infect human cerebral organoids and are transformed to bradyzoites and replicate in parasitophorous vacuoles to form cysts, indicating that the T. gondii asexual life cycle is efficiently simulated in the brain organoids.

Tue Dec 01 00:00:00 UTC 2020

Modelling Toxoplasma gondii infection in human cerebral organoids.

Emerging microbes & infections

Seo HH,Han HW,Lee SE,Hong SH,Cho SH,Kim SC,Koo SK,Kim JH

Tue Dec 01 00:00:00 UTC 2020

Modelling Toxoplasma gondii infection in human cerebral organoids.

Emerging microbes & infections

Seo HH,Han HW,Lee SE,Hong SH,Cho SH,Kim SC,Koo SK,Kim JH

Tue Dec 01 00:00:00 UTC 2020

Effect of Ionizing Radiation from Computed Tomography on Differentiation of Human Embryonic Stem Cells into Neural Precursors.

International journal of molecular sciences

Hanu C,Loeliger BW,Panyutin IV,Maass-Moreno R,Wakim P,Pritchard WF,Neumann RD,Panyutin IG

Published figure using PAX6 monoclonal antibody (Product # MA1-109) in Western Blot

Sat Aug 10 00:00:00 UTC 2019

Organoid single-cell genomic atlas uncovers human-specific features of brain development.

Nature

Kanton S,Boyle MJ,He Z,Santel M,Weigert A,Sanchís-Calleja F,Guijarro P,Sidow L,Fleck JS,Han D,Qian Z,Heide M,Huttner WB,Khaitovich P,Pääbo S,Treutlein B,Camp JG

MA1-109 was used in Immunohistochemistry to provide a temporal cell atlas of great ape forebrain development, and illuminate dynamic gene-regulatory features that are unique to humans.

Tue Oct 01 00:00:00 UTC 2019

Bioinformatics

Protein Aliases: Aniridia; Aniridia type II protein; Dickie's small eye; Keratitis; MGC17209; Oculorhombin; paired box gene 6; Paired Box Gene-6; paired box homeotic gene-6; Paired box protein Pax-6; small eye

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Gene Aliases: 1500038E17Rik; AEY11; AN; AN2; D11S812E; Dey; FVH1; Gsfaey11; MGDA; Pax-6; PAX6; Sey; WAGR

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UniProt ID: (Human) P26367, (Mouse) P63015

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Entrez Gene ID: (Human) 5080, (Mouse) 18508

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding ubiquitin-protein transferase activity protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding histone acetyltransferase binding co-SMAD binding R-SMAD binding HMG box domain binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding AT DNA binding double-stranded DNA binding sequence-specific DNA binding transcription regulatory region DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter establishment of mitotic spindle orientation blood vessel development eye development cell fate determination neuron migration negative regulation of protein phosphorylation positive regulation of neuroblast proliferation lens development in camera-type eye type B pancreatic cell differentiation pancreatic A cell development transcription from RNA polymerase II promoter smoothened signaling pathway axon guidance central nervous system development salivary gland morphogenesis visual perception response to wounding regulation of asymmetric cell division organ morphogenesis dorsal/ventral axis specification positive regulation of gene expression protein ubiquitination oligodendrocyte cell fate specification cerebral cortex regionalization forebrain anterior/posterior pattern specification forebrain dorsal/ventral pattern formation commitment of neuronal cell to specific neuron type in forebrain forebrain-midbrain boundary formation regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification regulation of transcription from RNA polymerase II promoter involved in somatic motor neuron fate commitment pituitary gland development signal transduction involved in regulation of gene expression keratinocyte differentiation regulation of cell migration positive regulation of epithelial cell differentiation lacrimal gland development protein localization to organelle eye photoreceptor cell development glucose homeostasis negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of timing of cell differentiation embryonic camera-type eye morphogenesis neuron fate commitment astrocyte differentiation negative regulation of epithelial cell proliferation negative regulation of neurogenesis retina development in camera-type eye iris morphogenesis cornea development in camera-type eye negative regulation of neural precursor cell proliferation neural crest cell migration epithelial cell development pancreatic A cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development axonogenesis brain development negative regulation of cell proliferation anterior/posterior pattern specification dorsal/ventral pattern formation regulation of gene expression regulation of neuron projection development pallium development rhombomere morphogenesis olfactory bulb development telencephalon regionalization interkinetic nuclear migration cell differentiation forebrain development hindbrain development positive regulation of cell fate specification camera-type eye development cell fate commitment regulation of neuron differentiation regulation of neurogenesis neural precursor cell proliferation positive regulation of glucagon secretion insulin metabolic process positive regulation of neuron migration

Performance Guarantee

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PAX6 Monoclonal Antibody (13B10-1A10)

PAX6 Antibody (MA1-109) in ICC/IF

Product Details

MA1-109

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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