Invitrogen

PAX6 Monoclonal Antibody (13B10-1A10)

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

3 Published Figures

5 References

View all (35) PAX6 antibodies

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Protocols

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PAX6 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of PAX6 (green) in human B3 lens epithelial cells (left panel) and negative control U-2 OS cells (right panel). The cells were fixed with formaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 1% Blocker BSA in PBS (Product # 37525) for 15 minutes, all at room temperature. Cells were stained with a PAX6 monoclonal antibody (Product # MA1-109) in 1% Blocker BSA in PBS (Product # 37525) at a dilution of 1:100 for 1 hour at room temperature, and then incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight-554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

PAX6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PAX6 showing staining in the nucleus of paraffin-embedded human pancreas tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a PAX6 monoclonal antibody (Product # MA1-109) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

PAX6 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunofluorescent analysis of PAX6 in human iPSC-derived forebrain organoids derived at Day 40. The organoids were fixed with 4% PFA for 1 hour at room temperature, followed by incubation with 30% sucrose solution overnight at 4°C. The organoids were then embedded in OCT and cryosectioned at 5 µm, permeabilized with 0.2% Triton X-100 for 20 min, and blocked with 10% donkey serum in PBS for 30 min at room temperature. Organoid slices were stained with a Mouse PAX6 monoclonal antibody (green; Product # MA1-109) at a dilution of 1:500 in blocking buffer overnight at 4°C, and then incubated with Donkey anti-Mouse Alexa Fluor 488 (Product # R37114) in blocking solution at room temperature for 1 hour. Images were taken at 20X magnification. Scale bar: 50 µm. Data courtesy of Dr. Zhexing Wen at Emory University.

PAX6 Antibody in Western Blot (WB) — Western blot was performed using Anti-PAX6 Monoclonal Antibody (13B10-1A10) (Product # MA1-109) and a 48 kDa band corresponding to PAX6 was observed in NCCIT cells on differentiation with retinoic acid. An additional uncharacterized band around 60 kDa (*) was observed in both lanes. Nuclear enriched extracts (30 µg lysate) of NCCIT (Lane 1) and NCCIT cells differentiated using retinoic acid treatment (10 µM for 10days) (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:20,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).

PAX6 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of Pax6 in human neural stem cells derived from PD-3 iPSCs using Gibco® PSC Neural Induction Medium (Product # A1647801). Cells were fixed, permeabilized, and then stained with a Pax6 monoclonal antibody (Product # MA1-109) at 1:100 dilution or mouse IgG1 isotype control (black histogram) in 5% BSA. After incubation of the primary antibody for 1 hour on ice, the cells were stained with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:500 for 1 hour on ice. A representative 10,000 cells were acquired for each sample.

PAX6 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Figure 1. Generation of cerebral brain organoids. (A) Schematic showing the method for generating hESC-derived brain organoids. (B) Representative images of a developing cerebral organoid at specific time points. (C) Immunohistochemistry of markers for the detection of neurons (SOX2 and TUJ1), radial glial cells (PAX6), astrocytes (GFAP) and oligodendrocytes (O1 and O4). Scale bars, as indicated. (D) Schematic representation of cerebral organoids generated in this study.

PAX6 Antibody in Immunohistochemistry (IHC) — Figure 1 OSVZ-expressed miR-137, but not miR-122, affects cortical progenitor proliferation (A) Microarray of the ferret ventricular zone (VZ), inner SVZ (iSVZ), and outer SVZ (oSVZ) during cortex development. Data are from and for post-natal day 2 (P2) and embryonic day 30 (E30)/E34/P2, respectively. Top left: schematic of microdissections of the P2 cortex performed for collection of P2 VZ, iSVZ, and oSVZ. Right: expression of miRNAs in the three germinal zones on P2. miR-122 and miR-137 cluster together as most expressed in the oSVZ. Bottom left: expression of miR-137 and miR-122 in the VZ and SVZ along development. (B) Progenitors in the VZ and SVZ at E16.5 upon miR-137 or miR-122 overexpression in the E14.5 mouse cortex. Top left: experimental design. Top right: quantification of KI67 + electroporated cells in the VZ and SVZ (scram, miR-137, and miR-122; n = 3 each). Bottom: representative micrographs of KI67 + electroporated cells. Electroporated cells positive (filled arrowheads) and negative (empty arrowheads) for KI67 are highlighted. (C) Neurogenic output on E17.5 upon miR-137 or miR-122 overexpression in the E14.5 mouse cortex. Top left: experimental design. Top right: quantification of NeuroD2 (ND2) + electroporated cells in the VZ and SVZ (scram, miR-137, and miR-122; n = 3 each). Bottom: representative micrographs of electroporated cells labeled for ND2. Electroporated cells positive (filled arrowheads) and negative (empty arrowheads) for ND2 are highlighted. (D)

PAX6 Antibody — The specificity of anti-PAX6 mouse monoclonal antibody (Product # MA1-109) was demonstrated by the immunofluorescence detection of Pax6 expression in positive human B3 lens epithelial cells (left panel) compared to negative control U-2OS cells (right panel). {RE}

Product Details

MA1-109

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	1:50-1:200	View 1 publication 1 publication
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	13B10-1A10
Immunogen	Purified internal fragment of human recombinant PAX6 expressed in E. coli.
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS with 1mg/mL BSA, 30% glycerol
Contains	0.05% sodium azide
Storage conditions	-20°C
RRID	AB_2536820

Product Specific Information

Western blot analysis of MA1-109 detects an ~47 kDa protein in whole cell lysates from early passage human B3 lens epithelial cells and in mouse eye lysate. An additional unknown band at ~36 kDa is also detected in B3 cell lysates. This antibody will detect additional nonspecific bands in lysates with very low basal PAX6 expression. Specificity of the antibody was confirmed in B3 and mouse eye lysates (positive controls) compared to U-2 OS human osteosarcoma and NIH/3T3 mouse embryonic fibroblasts (negative controls).

MA1-109- can be used for immunofluoresence and flow cytometry analysis of Pax6 in human neural stem cells.

Target Information

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

miR-137 and miR-122, two outer subventricular zone non-coding RNAs, regulate basal progenitor expansion and neuronal differentiation.

Cell reports

Tomasello U,Klingler E,Niquille M,Mule N,Santinha AJ,de Vevey L,Prados J,Platt RJ,Borrell V,Jabaudon D,Dayer A

MA1-109 was used in Immunohistochemistry to support a cell-type-specific role of miRNA-mediated gene expression in cortical expansion.

Tue Feb 15 00:00:00 EST 2022

miR-137 and miR-122, two outer subventricular zone non-coding RNAs, regulate basal progenitor expansion and neuronal differentiation.

Cell reports

Tomasello U,Klingler E,Niquille M,Mule N,Santinha AJ,de Vevey L,Prados J,Platt RJ,Borrell V,Jabaudon D,Dayer A

MA1-109 was used in Immunohistochemistry to support a cell-type-specific role of miRNA-mediated gene expression in cortical expansion.

Tue Feb 15 00:00:00 EST 2022

Glycoproteomic analysis of the changes in protein N-glycosylation during neuronal differentiation in human-induced pluripotent stem cells and derived neuronal cells.

Scientific reports

Kimura K,Koizumi T,Urasawa T,Ohta Y,Takakura D,Kawasaki N

MA1-109 was used in Western Blot to conduct sequential LC/MS/MS analyses of tryptic digest, enriched glycopeptides, and deglycosylated peptides of proteins derived from human-induced pluripotent stem cells (iPSCs) and iPSC-derived neuronal cells.

Thu May 27 00:00:00 EDT 2021

Modelling Toxoplasma gondii infection in human cerebral organoids.

Emerging microbes & infections

Seo HH,Han HW,Lee SE,Hong SH,Cho SH,Kim SC,Koo SK,Kim JH

MA1-109 was used in Immunocytochemistry to show that tachyzoites can infect human cerebral organoids and are transformed to bradyzoites and replicate in parasitophorous vacuoles to form cysts, indicating that the T. gondii asexual life cycle is efficiently simulated in the brain organoids.

Tue Dec 01 00:00:00 EST 2020

Modelling Toxoplasma gondii infection in human cerebral organoids.

Emerging microbes & infections

Seo HH,Han HW,Lee SE,Hong SH,Cho SH,Kim SC,Koo SK,Kim JH

Tue Dec 01 00:00:00 EST 2020

Modelling Toxoplasma gondii infection in human cerebral organoids.

Emerging microbes & infections

Seo HH,Han HW,Lee SE,Hong SH,Cho SH,Kim SC,Koo SK,Kim JH

Tue Dec 01 00:00:00 EST 2020

Effect of Ionizing Radiation from Computed Tomography on Differentiation of Human Embryonic Stem Cells into Neural Precursors.

International journal of molecular sciences

Hanu C,Loeliger BW,Panyutin IV,Maass-Moreno R,Wakim P,Pritchard WF,Neumann RD,Panyutin IG

Published figure using PAX6 monoclonal antibody (Product # MA1-109) in Western Blot

Sat Aug 10 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: Aniridia; Aniridia type II protein; Dickie's small eye; Keratitis; MGC17209; Oculorhombin; paired box gene 6; Paired Box Gene-6; paired box homeotic gene-6; Paired box protein Pax-6; small eye

Gene Aliases: 1500038E17Rik; AEY11; AN; AN2; D11S812E; Dey; FVH1; Gsfaey11; MGDA; Pax-6; PAX6; Sey; WAGR

UniProt ID: (Human) P26367, (Mouse) P63015

Entrez Gene ID: (Human) 5080, (Mouse) 18508

Function(s)

negative regulation of transcription from RNA polymerase II promoter establishment of mitotic spindle orientation blood vessel development eye development cell fate determination neuron migration negative regulation of protein phosphorylation positive regulation of neuroblast proliferation lens development in camera-type eye type B pancreatic cell differentiation pancreatic A cell development transcription from RNA polymerase II promoter smoothened signaling pathway axon guidance central nervous system development salivary gland morphogenesis visual perception response to wounding regulation of asymmetric cell division organ morphogenesis dorsal/ventral axis specification positive regulation of gene expression protein ubiquitination oligodendrocyte cell fate specification cerebral cortex regionalization forebrain anterior/posterior pattern specification forebrain dorsal/ventral pattern formation commitment of neuronal cell to specific neuron type in forebrain forebrain-midbrain boundary formation regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification regulation of transcription from RNA polymerase II promoter involved in somatic motor neuron fate commitment pituitary gland development signal transduction involved in regulation of gene expression keratinocyte differentiation regulation of cell migration positive regulation of epithelial cell differentiation lacrimal gland development protein localization to organelle eye photoreceptor cell development glucose homeostasis negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of timing of cell differentiation embryonic camera-type eye morphogenesis neuron fate commitment astrocyte differentiation negative regulation of epithelial cell proliferation negative regulation of neurogenesis retina development in camera-type eye iris morphogenesis cornea development in camera-type eye negative regulation of neural precursor cell proliferation neural crest cell migration epithelial cell development pancreatic A cell differentiation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter multicellular organism development axonogenesis brain development negative regulation of cell proliferation anterior/posterior pattern specification dorsal/ventral pattern formation regulation of gene expression regulation of neuron projection development pallium development rhombomere morphogenesis olfactory bulb development telencephalon regionalization interkinetic nuclear migration cell differentiation forebrain development hindbrain development positive regulation of cell fate specification camera-type eye development cell fate commitment regulation of neuron differentiation regulation of neurogenesis neural precursor cell proliferation positive regulation of glucagon secretion insulin metabolic process positive regulation of neuron migration

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding ubiquitin-protein transferase activity protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding histone acetyltransferase binding co-SMAD binding R-SMAD binding HMG box domain binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding AT DNA binding double-stranded DNA binding sequence-specific DNA binding transcription regulatory region DNA binding

PAX6 Monoclonal Antibody (13B10-1A10)

Product Details

MA1-109

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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