Rockland

PAX6 Monoclonal Antibody (7H10.D3.D4.G5)

View all (44) PAX6 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Details

200-301-R22

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:100

ELISA (ELISA)

1:75,000-1:150,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

7H10.D3.D4.G5

Immunogen

PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to human PAX6 protein.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Cross-reactivity with PAX6 from other sources has not been determined.

Target Information

This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Aniridia; Aniridia type II protein; Keratitis; MGC17209; Oculorhombin; Paired Box Gene-6; paired box homeotic gene-6; Paired box protein Pax-6

Gene Aliases: AN; AN2; D11S812E; FVH1; MGDA; PAX6; WAGR

UniProt ID: (Human) P26367

Entrez Gene ID: (Human) 5080

Function(s)

negative regulation of transcription from RNA polymerase II promoter establishment of mitotic spindle orientation blood vessel development eye development cell fate determination neuron migration negative regulation of protein phosphorylation positive regulation of neuroblast proliferation lens development in camera-type eye type B pancreatic cell differentiation pancreatic A cell development transcription from RNA polymerase II promoter smoothened signaling pathway axon guidance central nervous system development salivary gland morphogenesis visual perception response to wounding regulation of asymmetric cell division organ morphogenesis dorsal/ventral axis specification positive regulation of gene expression protein ubiquitination oligodendrocyte cell fate specification cerebral cortex regionalization forebrain anterior/posterior pattern specification forebrain dorsal/ventral pattern formation commitment of neuronal cell to specific neuron type in forebrain forebrain-midbrain boundary formation regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification regulation of transcription from RNA polymerase II promoter involved in somatic motor neuron fate commitment pituitary gland development signal transduction involved in regulation of gene expression keratinocyte differentiation regulation of cell migration positive regulation of epithelial cell differentiation lacrimal gland development protein localization to organelle eye photoreceptor cell development glucose homeostasis negative regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of timing of cell differentiation embryonic camera-type eye morphogenesis neuron fate commitment astrocyte differentiation negative regulation of epithelial cell proliferation negative regulation of neurogenesis retina development in camera-type eye iris morphogenesis cornea development in camera-type eye negative regulation of neural precursor cell proliferation

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding ubiquitin-protein transferase activity protein binding transcription factor binding protein kinase binding ubiquitin protein ligase binding histone acetyltransferase binding co-SMAD binding R-SMAD binding HMG box domain binding