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PBRM1 Antibody (MA5-37814) in WB
Western blot analysis of PBRM1 in extracts of various cell lines. Samples were incubated with PBRM1 Monoclonal antibody (Product # MA5-37814) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
MA5-37814
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC1820
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 530-680 of human PBRM1 (Q86U86)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2897738
Product Specific Information
Positive Samples: HeLa, NIH/3T3, Jurkat, Rat brain
Immunogen sequence: VLEAREPGSG RRLCDLFMVK PSKKDYPDYY KIILEPMDLK IIEHNIRNDK YAGEEGMIED MKLMFRNARH YNEEGSQVYN DAHILEKLLK EKRKELGPLP DDDDMASPKL KLSRKSGISP KKSKYMTPMQ QKLNEVYEAV KNYTDKRGRR L
Target Information
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2310032M22Rik; BAF180; BRG1-associated factor 180; hPB1; polybromo 1; Polybromo-1D; Protein polybromo-1
Gene Aliases: 2610016F04Rik; AI507524; BAF180; PB1; PBRM1; RGD1565549
UniProt ID: (Human) Q86U86
Entrez Gene ID: (Human) 55193, (Rat) 306254, (Mouse) 66923
Molecular Function:
chromatin/chromatin-binding, or -regulatory protein
chromatin remodeling
transcription, DNA-templated
regulation of transcription, DNA-templated
mitotic nuclear division
negative regulation of cell proliferation
covalent chromatin modification
placenta development
heart development
biological_process
blood vessel remodeling
heart morphogenesis
epicardium-derived cardiac endothelial cell differentiation
chromatin modification
positive regulation of transcription, DNA-templated
cardiac vascular smooth muscle cell development
vasculogenesis involved in coronary vascular morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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