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|Tested species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues S(111) V Q K D S A L D L F N D P M W N(127) of mouse PC1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-057 detects prohormone convertase 1 (PC1) from mouse pituitary extract.
PA1-057 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~80 kDa protein representing PC1 from mouse pituitary extract.
The PA1-057 immunogen is a synthetic peptide corresponding to residues S(111) V Q K D S A L D L F N D P M W N(127) of mouse PC1. This sequence is 88% and 82% conserved bovine and rat, respectively. The PA1-057 immunizing peptide (Cat. # PEP-164) is available for use in neutralization and control experiments.
The subtilisin-like prohormone convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Obesity, hyperphagia and increased metabolic efficiency in Pc1 mutant mice.
PA1-057 was used in western blot to investigate the roles of prohormone convertase 1 in obesity, abnormal proinsulin processing and multiple endocrinological defects.
|Lloyd DJ,Bohan S,Gekakis N||Human molecular genetics (15:1884)||2006|
EC 126.96.36.199; furin homolog; NEC 1; neuroendocrine convertase 1; Prohormone Convertase 1; prohormone convertase 1/3; propeptide-processing protease; proprotein convertase 1
Att-1; Nec-1; Nec1; PC1; PC3; Pcsk1; Phpp-1; SPC3