|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human PCNA (amino acids: 53-196 ) expressed in E. Coli.|
|Storage buffer||PBS with 0.05% proprietary stabilizer|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200 - 1:400|
|Immunohistochemistry (IHC)||1:200 - 1:1000|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-17145 targets PCNA in FACS, IHC, and WB applications and shows reactivity with Human samples.
The MA5-17145 immunogen is purified recombinant fragment of human PCNA (amino acids: 53-196 ) expressed in E. Coli.
MA5-17145 detects PCNA which has a predicted molecular weight of approximately 28.7kDa.
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.