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|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 230 and 290 of CD274|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-28115 targets CD274 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-28115 immunogen is a synthetic peptide corresponding to a region within amino acids 230 and 290 of CD274.
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Essential role of HDAC6 in the regulation of PD-L1 in melanoma.
PA5-28115 was used in western blot to characterize regulation of PD-L1 in melanoma by HDAC6
|Lienlaf M,Perez-Villarroel P,Knox T,Pabon M,Sahakian E,Powers J,Woan KV,Lee C,Cheng F,Deng S,Smalley KS,Montecino M,Kozikowski A,Pinilla-Ibarz J,Sarnaik A,Seto E,Weber J,Sotomayor EM,Villagra A||Molecular oncology (10:735)||2016|
B7 homolog 1; B7-H; B7-H1; B7H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; PDL1; Programmed cell death 1 ligand 1; programmed death ligand 1
B7-H; B7H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1