NeoBiotechnologies

PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target) Monoclonal Antibody (PDL1, 2746)

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Datasheet

Protocols

Questions & Answers

Tech Support

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PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target) Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of Jurkat cells using PD-L1, PDCD1LG1, CD274, B7-H1 (Cancer Immunotherapy Target) Monoclonal Antibody (Product # 29126-MSM6-P1) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).

PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human basal cell carcinoma using PD-L1, PDCD1LG1, CD274, B7-H1 (Cancer Immunotherapy Target) Monoclonal Antibody (Product # 29126-MSM6-P1).

PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target) Antibody in Western Blot (WB) — Western Blot analysis of RAW, A549, THP1, MOLT-4, Raji, and MDA-MB-231 cell lysates using PD-L1, PDCD1LG1, CD274, B7-H1 (Cancer Immunotherapy Target) Monoclonal Antibody (Product # 29126-MSM6-P1).

PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target) Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of human Jurkat cells using PD-L1, PDCD1LG1, CD274, B7-H1 (Cancer Immunotherapy Target) Monoclonal Antibody (Product # 29126-MSM6-P1) followed by goat anti-Mouse IgG-CF488 (Orange); cells alone (Blue); Isotype Control (Red).

PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target) Antibody in Peptide array (ARRAY) — Analysis of Protein Array containing more than 19,000 full-length human proteins PD-L1, PDCD1LG1, CD274, B7-H1 (Cancer Immunotherapy Target) Monoclonal Antibody (Product # 29126-MSM6-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDÔøΩs) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDÔøΩs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target) Antibody in SDS-PAGE (SDS-PAGE) — SDS-PAGE Analyis Purified PD-L1, PDCD1LG1, CD274, B7-H1 (Cancer Immunotherapy Target) Monoclonal Antibody (Product # 29126-MSM6-P1). Confirmation of Purity and Integrity of Antibody.

Product Details

29126-MSM6-P1

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Immunocytochemistry (ICC/IF)

1-2 µg/mL

Flow Cytometry (Flow)

1-2 µg/10^6 cells

Peptide Array (Array)

Assay-dependent

SDS-PAGE (SDS-PAGE)

Assay-dependent

Product Specifications

Species Reactivity

Mouse, Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

PDL1, 2746

Immunogen

Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

200 µg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

4° C

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B7 homolog 1; B7-H1; CD274; CD274 antigen; PD-L1; PDCD1 ligand 1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; sCD274; soluble CD274; soluble PD L1; sPD L1

Gene Aliases: A530045L16Rik; B7-H; B7H1; CD274; PD-L1; PDCD1L1; PDCD1LG1; PDL1

UniProt ID: (Human) Q9NZQ7, (Mouse) Q9EP73

Entrez Gene ID: (Human) 29126, (Mouse) 60533

Molecular Function: immunoglobulin receptor superfamily