Invitrogen

PDE4A Monoclonal Antibody (OTI6B6)

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PDE4A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of PDE4A in COS7 cells. Cells were transfected with a plasmid overexpressing PDE4A and probed with a PDE4A monoclonal antibody (Product # MA5-25109).

PDE4A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on paraffin-embedded prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a PDE4A monoclonal antibody (Product # MA5-25109) at a dilution of 1:50.

PDE4A Antibody in Western Blot (WB) — Western blot analysis of PDE4A in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with PDE4A (Product # MA5-25109) monoclonal antibody.

Product Details

MA5-25109

Applications	Tested Dilution	Publications
Western Blot (WB)	1:4,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI6B6
Immunogen	Full length human recombinant protein of PDE4A produced in HEK293T cell View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 1% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2724970

Target Information

Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. Cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. PDE4A is a class IV cAMP-specific PDE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cAMP-specific 3',5'-cyclic phosphodiesterase 4A; DPDE2; PDE46; phosphodiesterase 4A, cAMP-specific (dunce; phosphodiesterase E2 dunce homolog, Drosophila; phosphodiesterase isozyme 4

Gene Aliases: DPDE2; PDE4; PDE46; PDE4A

UniProt ID: (Human) P27815

Entrez Gene ID: (Human) 5141

Molecular Function: phosphodiesterase