|Tested species reactivity||Bovine, Sheep|
|Published species reactivity||Bovine, Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: M(1) G E V T A E E V E K F L D S N(16) C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1-2 µg/ml|
PA1-720 detects phosphodiesterase 6 alpha (PDE6 alpha) from sheep retinal extract and bovine rod outer segment membranes as well as purified bovine PDE6.
PA1-720 has been successfully used in Western blot and immunofluoresence procedures. By Western blot, this antibody detects an ~90 kDa protein representing PDE6 alpha from sheep retinal extract. A higher molecular weight band is sometimes observed using sheep retinal extracts.
The PA1-720 immunizing peptide corresponds to amino acid residues 1-16 from human PDE6 alpha. This sequence is completely conserved between human, mouse and bovine and 94% conserved in canine. PA1-720 immunizing peptide (Cat. # PEP-148) is available for use in neutralization and control experiments.
This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.
Essential role of the chaperonin CCT in rod outer segment biogenesis.
PA1-720 was used in immunocytochemistry to study the biogenesis of rod outer segments and the indespensible role of chaperonin containing t-complex protein-1
|Sinha S,Belcastro M,Datta P,Seo S,Sokolov M||Investigative ophthalmology & visual science (55:3775)||2014|
Leukemia inhibitory factor coordinates the down-regulation of the visual cycle in the retina and retinal-pigmented epithelium.
PA1-720 was used in western blot to study the role of leukemia inhibitory factor on the visual cycle in the retina and retinal-pigmented epithelium
|Chucair-Elliott AJ,Elliott MH,Wang J,Moiseyev GP,Ma JX,Politi LE,Rotstein NP,Akira S,Uematsu S,Ash JD||The Journal of biological chemistry (287:24092)||2012|
Effect of the ILE86TER mutation in the γ subunit of cGMP phosphodiesterase (PDE6) on rod photoreceptor signaling.
PA1-720 was used in western blot to investigate the importance of PDE6 gamma in rod photoreceptor signaling
|Tsang SH,Woodruff ML,Lin CS,Jacobson BD,Naumann MC,Hsu CW,Davis RJ,Cilluffo MC,Chen J,Fain GL||Cellular signalling (24:181)||2012|
Function of the asparagine 74 residue of the inhibitory γ-subunit of retinal rod cGMP-phophodiesterase (PDE) in vivo.
PA1-720 was used in western blot to investigate the effect of N74A mutation on the function of PDE6 in vivo
|Tsang SH,Woodruff ML,Hsu CW,Naumann MC,Cilluffo M,Tosi J,Lin CS||Cellular signalling (23:1584)||2011|
Replacing the rod with the cone transducin subunit decreases sensitivity and accelerates response decay.
PA1-720 was used in western blot to investigate the mechanism why cone vision is less sensitive than rod vision
|Chen CK,Woodruff ML,Chen FS,Shim H,Cilluffo MC,Fain GL||The Journal of physiology (588:3231)||2010|
High levels of retinal membrane docosahexaenoic acid increase susceptibility to stress-induced degeneration.
PA1-720 was used in western blot to study the effect of retinal membrane docosahexaenoic acid on stress-induced retinal degeneration in mice.
|Tanito M,Brush RS,Elliott MH,Wicker LD,Henry KR,Anderson RE||Journal of lipid research (50:807)||2009|
Phototransduction in a transgenic mouse model of Nougaret night blindness.
PA1-720 was used in western blot to investigate the mechanism for Nougaret night blindness.
|Moussaif M,Rubin WW,Kerov V,Reh R,Chen D,Lem J,Chen CK,Hurley JB,Burns ME,Artemyev NO||The Journal of neuroscience : the official journal of the Society for Neuroscience (26:6863)||2006|
The inhibitory gamma subunit of the rod cGMP phosphodiesterase binds the catalytic subunits in an extended linear structure.
PA1-720 was used in western blot to study the interaction between the inhibitory gamma subunit and the catalytic subunit of the rod cGMP phosphodiesterase.
|Guo LW,Muradov H,Hajipour AR,Sievert MK,Artemyev NO,Ruoho AE||The Journal of biological chemistry (281:15412)||2006|
GAP-independent termination of photoreceptor light response by excess gamma subunit of the cGMP-phosphodiesterase.
PA1-720 was used in western blot to study the effect of the cGMP-phosphodiesterase gamma subunit on photoreceptor light response termination.
|Tsang SH,Woodruff ML,Chen CK,Yamashita CY,Cilluffo MC,Rao AL,Farber DB,Fain GL||The Journal of neuroscience : the official journal of the Society for Neuroscience (26:4472)||2006|
Transducin activation state controls its light-dependent translocation in rod photoreceptors.
PA1-720 was used in western blot to investigate the role of the GTP-hydrolysis on Gtalpha for light-dependent translocation in rod photoreceptors
|Kerov V,Chen D,Moussaif M,Chen YJ,Chen CK,Artemyev NO||The Journal of biological chemistry (280:41069)||2005|
Asymmetric interaction between rod cyclic GMP phosphodiesterase gamma subunits and alphabeta subunits.
PA1-720 was used in western blot to demonstrate a new model of interaction between the subunits of the rod cyclic GMP phosphodiesterase holoenzyme.
|Guo LW,Grant JE,Hajipour AR,Muradov H,Arbabian M,Artemyev NO,Ruoho AE||The Journal of biological chemistry (280:12585)||2005|
|Bovine||1:500 to 1:1,000||
Enhancement of phototransduction g protein-effector interactions by phosphoinositides.
PA1-720 was used in western blot to study the modulating mechanism for phototransduction efficiency.
|He F,Mao M,Wensel TG||The Journal of biological chemistry (279:8986)||2004|
Identification of the gamma subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6alpha '.
PA1-720 was used in western blot to investigate the regulation of photoreceptor cGMP phosphodiesteras 6 activity
|Granovsky AE,Artemyev NO||The Journal of biological chemistry (275:41258)||2000|
In vitro interaction of tubulin with the photoreceptor cGMP phosphodiesterase gamma-subunit.
PA1-720 was used in immunohistochemistry to study the interaction of the photoreceptor cGMP phosphodiesterase with tubulin.
|Chu UB,Song J,Mavlyutov TA,Guo LW||Neuroscience letters (482:225)||2010|
cGMP-Specific Rod -Alpha; GMP-PDE alpha; PDE V-B1; Phosphodiesterase 6 Alpha; phosphodiesterase 6A; phosphodiesterase 6A, cGMP-specific, rod, alpha; phosphodiesterase, cyclic GMP (rod receptor), alpha polypeptide; rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
BOS_8294; PDE6A; PDEA