|Tested species reactivity||Bovine, Human, Sheep|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues M(16) P Q F A K E Y F D R K L R(29) C of human PDE6 alpha and quote;.|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
PA1-721 detects phosphodiesterase 6 alpha and quote; (PDE6 alpha and quote;) from sheep, human and bovine retinal extract.
PA1-721 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~90 kDa protein representing PDE6 alpha and quote; from sheep and bovine retinal extract.
The PA1-721 immunogen is a synthetic peptide corresponding to residues M(16) P Q F A K E Y F D R K L R(29) C of human PDE6 alpha and quote;. This sequence is completely conserved between human, chicken and frog. PA1-721 immunizing peptide (Cat. # PEP-149) is available for use in neutralization and control experiments.
This gene encodes the cyclic-GMP (cGMP)-specific phosphodiesterase 6A alpha subunit, expressed in cells of the retinal rod outer segment. The phosphodiesterase 6 holoenzyme is a heterotrimer composed of an alpha, beta, and two gamma subunits. cGMP is an important regulator of rod cell membrane current, and its dynamic concentration is established by phosphodiesterase 6A cGMP hydrolysis and guanylate cyclase cGMP synthesis. The protein is a subunit of a key phototransduction enzyme and participates in processes of transmission and amplification of the visual signal. Mutations in this gene have been identified as one cause of autosomal recessive retinitis pigmentosa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Active 3'-5' cyclic nucleotide phosphodiesterases are present in detergent-resistant membranes of mural granulosa cells.
PA1-721 was used in immunocytochemistry and western blot to analyze detergent-resistant membranes of mural granulosa cells and the presence of active 3'-5' cyclic nucleotide phosphodiesterases
|Bergeron A,Guillemette C,Sirard MA,Richard FJ||Reproduction, fertility, and development (null:null)||2016|