Immunofluorescent analysis of PDE6 delta (green) showing staining in the cytoplasm and nucleus of BAEC cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a PDE6 delta polyclonal antibody (Product # PA1-726) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Human, Sheep|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptides: M(1) S A K D E R A R (D/E) I L R G F K(16) C|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1.0 ug/ml|
|Western Blot (WB)||2.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 1 publications below|
PA1-726 detects PrBP/delta from bovine, human and sheep samples.
PA1-726 has been successfully used in Western blot, ICC/IF and immunohistochemistry procedures. By Western blot, this antibody detects a 17 kDa protein representing PrBP/delta from bovine and sheep retinal/optic nerve extracts as well as HeLa cell lysate. Immunohistochemical staining of PrBP/delta in bovine retinal cross-sections using PA1-726 results in staining of retinal outer segments.
PA1-726 immunizing peptides correspond to amino acid residues 1-16 from mouse and human PrBP/delta. This peptide
(Cat. # PEP-203) is available for use in neutralization and control experiments.
PrBP/delta, previously known as PDE6/delta, is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). PRBP/delta has been implicated in the transport and membrane targeting of prenylated proteins (including PDE6) from their site of synthesis in the inner segment to their final destination in the outer segment of rods and cones (Norton et. al., 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mammalian sperm phosphodiesterases and their involvement in receptor-mediated cell signaling important for capacitation.
PA1-726 was used in immunocytochemistry to investigate the expression and function of different PDE isoforms in mature mouse spermatozoa
|Baxendale RW,Fraser LR||Molecular reproduction and development (71:495)||2005|