|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GST fusion protein containing a carboxy-terminal fragment of human PDGF receptor ß|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.
IP-MS enrichment of PDGFRB (LFQ intensity): PDGFRB was enriched 83-fold from NIH/3T3 lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Part No. 90409) and PDGFRB antibody (Part No. MA5-15143). The STRING database (www.string-db.org) was used to identify the protein interactor list. See more information on IP-MS verification of antibody selectivity. IP-MS validation info.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Osteogenic potential of alpha smooth muscle actin expressing muscle resident progenitor cells.
MA5-15143 was used in immunohistochemistry to characterize alpha smooth muscle actin for osteogenic potential by expression muscle resident progenitor cells
|Matthews BG,Torreggiani E,Roeder E,Matic I,Grcevic D,Kalajzic I||Bone (84:69)||2016|
Bile acids induce hepatic differentiation of mesenchymal stem cells.
MA5-15143 was used in immunocytochemistry to study the effect of bile acids on mesenchymal stem cells in liver
|Sawitza I,Kordes C,Götze S,Herebian D,Häussinger D||Scientific reports (5:null)||2015|
Hepatic stellate cells support hematopoiesis and are liver-resident mesenchymal stem cells.
MA5-15143 was used in immunocytochemistry to study the role of hepatic stellate cells as liver-resident mesenchymal stem cells and their role in hematopoiesis
|Kordes C,Sawitza I,Götze S,Häussinger D||Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (31:290)||2013|