Immunohistochemistry analysis of PDGFR alpha showing staining in the membrane and cytoplasm of paraffin-embedded mouse kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a PDGFR alpha Rabbit Polyclonal Antibody (PA516742) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from C-terminal of human PDGFR alpha|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16742 targets PDGFR alpha in IHC (P) applications and shows reactivity with Human and mouse samples.
The PA5-16742 immunogen is a synthetic peptide derived from C-terminal of human PDGFR alpha.
PDGFR (Platelet Derived Growth Factor Receptor) exhibits two different isoforms, alpha; and beta; coded by two different genes. PDGF A receptors are expressed in oligodendrocyte progenitor cells and PDGF B receptors are expressed on neurons. This antibody is specific for PDGFR, alpha; and does not react with PDGFR, beta;.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.