Immunofluorescent analysis of PDGFR alpha in HeLa cells using a PDGFR alpha recombinant rabbit monoclonal antibody (Product # 701142) followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic and nuclear membrane localization of PDGFR-alpha.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 259–436 of human platelet-derived growth factor receptor – alpha polypeptide|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1 ul|
|Immunocytochemistry (ICC)||1-3 ug/ml|
|Immunofluorescence (IF)||1-3 ug/ml|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
PDGFR-alpha belongs to the protein tyrosine kinase family, and binds to the alpha chain of PDGF. Members of the PDGF receptor subfamily are cell surface receptors that act as mitogens for cells of mesenchymal origin, and consist of alpha and beta isoforms of ~190, and 125 kDa, respectively. Activated PDGF receptors act on signaling pathways that control cell growth, actin reorganization, migration, and differentiation. In humans, the gene is located on the q arm of chromosome 4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.