|Tested species reactivity||Dog, Hamster, Human, Mouse, Pig, Rat, Xenopus|
|Published species reactivity||Rat, Pig, Non-human primate, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Purified rat PDI protein.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay dependent|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:200-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 14 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Western Blot (WB)||See 7 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
MA3-018 detects protein disulphide-isomerase (PDI) from human, mouse, rat, porcine, xenopus liver tissues as well as hamster (CHO) cells and canine samples.
MA3-018 has been successfully used in Western blot, immunofluorescence, immunohistochemical, flow cytometry, and immunoprecipitation procedures. By Western blot, this antibody detects a 59 kDa protein representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract. Immunohistochemical staining of PDI in rat intestine with MA3-018 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures MA3-018 has been shown to inhibit the activity of PDI in vitro.
In immunohistochemistry procedures, formalin-fixed paraffin-embedded tissue sections are recommended.
The MA3-018 antigen is purified rat PDI.
The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide-Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
ER-mitochondria associations are regulated by the VAPB-PTPIP51 interaction and are disrupted by ALS/FTD-associated TDP-43.
MA3-018 was used in immunocytochemistry to study ER-mitochondria associations disrupted by ALS/FTD-associated TDP-43 and regulated by VAPB-PTPIP51 interactions
|Stoica R,De Vos KJ,Paillusson S,Mueller S,Sancho RM,Lau KF,Vizcay-Barrena G,Lin WL,Xu YF,Lewis J,Dickson DW,Petrucelli L,Mitchell JC,Shaw CE,Miller CC||Nature communications (5:null)||2014|
Virus-tumor interactome screen reveals ER stress response can reprogram resistant cancers for oncolytic virus-triggered caspase-2 cell death.
MA3-018 was used in immunocytochemistry to investigate the effect of ER stress response on oncolytic virus-mediated cancer therapy
|Mahoney DJ,Lefebvre C,Allan K,Brun J,Sanaei CA,Baird S,Pearce N,Grönberg S,Wilson B,Prakesh M,Aman A,Isaac M,Mamai A,Uehling D,Al-Awar R,Falls T,Alain T,Stojdl DF||Cancer cell (20:443)||2011|
GULP1 is a novel APP-interacting protein that alters APP processing.
MA3-018 was used in immunocytochemistry to investigate the role of GULP1 in APP processing and A beta peptide accumulation
|Hao Y,Hao CY,Perkinton MS,Chan WW,Chan HY,Miller CC,Lau KF||The Biochemical journal (436:631)||2011|
|Non-human primate||Not Cited||
Mouse RIC-3, an endoplasmic reticulum chaperone, promotes assembly of the alpha7 acetylcholine receptor through a cytoplasmic coiled-coil domain.
MA3-018 was used in immunocytochemistry to study the role of the endoplasmic reticulum chaperone RIC-3 in the alpha7 acetylcholine receptor assembly.
|Wang Y,Yao Y,Tang XQ,Wang ZZ||The Journal of neuroscience : the official journal of the Society for Neuroscience (29:12625)||2009|
A dual task for the Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum: inhibiting secretion of misfolded protein conformers and enhancing their disposal.
MA3-018 was used in immunocytochemistry to investigate the role of Xbp1-responsive OS-9 variants in mammalian ER quality control
|Bernasconi R,Pertel T,Luban J,Molinari M||The Journal of biological chemistry (283:16446)||2008|
The Niemann-Pick C1 gene is downregulated by feedback inhibition of the SREBP pathway in human fibroblasts.
MA3-018 was used in immunocytochemistry to study the regulation of Niemann-Pick C1 gene in human fibroblasts
|Garver WS,Jelinek D,Francis GA,Murphy BD||Journal of lipid research (49:1090)||2008|
An aberrant sequence in a connexin46 mutant underlies congenital cataracts.
MA3-018 was used in immunocytochemistry to investigate the role of connexin46 mutations in congenital cataracts.
|Minogue PJ,Liu X,Ebihara L,Beyer EC,Berthoud VM||The Journal of biological chemistry (280:40788)||2005|
Analysis of foot-and-mouth disease virus internalization events in cultured cells.
MA3-018 was used in immunocytochemistry to study the internalization of foot-and-mouth disease virus in cultured human cells
|O'Donnell V,LaRocco M,Duque H,Baxt B||Journal of virology (79:8506)||2005|
Postsynaptic expression of homeostatic plasticity at neocortical synapses.
MA3-018 was used in immunocytochemistry to investigate the expression of synaptic AMPA receptors, glutamate receptors GluR1 and GluR2 in visual cortical cultures.
|Wierenga CJ,Ibata K,Turrigiano GG||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:2895)||2005|
Constitutive and agonist-dependent self-association of the cell surface human lutropin receptor.
MA3-018 was used in immunocytochemistry to investigate the role of human lutropin receptor in G protein-coupled receptor dimerization and oligomerization.
|Tao YX,Johnson NB,Segaloff DL||The Journal of biological chemistry (279:5904)||2004|
ERp19 and ERp46, new members of the thioredoxin family of endoplasmic reticulum proteins.
MA3-018 was used in immunocytochemistry to characterize two new endoplasmic reticulum thioredoxin family members ERp19 and ERp46
|Knoblach B,Keller BO,Groenendyk J,Aldred S,Zheng J,Lemire BD,Li L,Michalak M||Molecular and cellular proteomics : MCP (2:1104)||2003|
Phenotypic differences between peripheral myelin protein-22 (PMP22) and myelin protein zero (P0) mutations associated with Charcot-Marie-Tooth-related diseases.
MA3-018 was used in immunocytochemistry to study phenotypic differences between peripheral myelin protein-22 and myelin protein zero mutations in Charcot-Marie-Tooth-related diseases
|Shames I,Fraser A,Colby J,Orfali W,Snipes GJ||Journal of neuropathology and experimental neurology (62:751)||2003|
|Non-human primate||Not Cited||
Formation of transitory intrachain and interchain disulfide bonds accompanies the folding and oligomerization of simian virus 40 Vp1 in the cytoplasm.
MA3-018 was used in immunocytochemistry to study the structural alteration and oligomerization of cytoplasmic simian virus 40 Vp1
|Li PP,Nakanishi A,Clark SW,Kasamatsu H||Proceedings of the National Academy of Sciences of the United States of America (99:1353)||2002|
|Human||5 ul/100 ul||
Sulfhydryl regulation of L-selectin shedding: phenylarsine oxide promotes activation-independent L-selectin shedding from leukocytes.
MA3-018 was used in immunocytochemistry to study the expression and the function of protein-disulfide isomerase.
|Bennett TA,Edwards BS,Sklar LA,Rogelj S||Journal of immunology (Baltimore, Md. : 1950) (164:4120)||2000|
|Mouse||35 ug/500 ug||
Cigarette smoking affects oxidative protein folding in endoplasmic reticulum by modifying protein disulfide isomerase.
MA3-018 was used in immunoprecipitation to study the modification of protein disulfide isomerase by cigarette smoke and the effect on endoplasmic reticulum oxidative protein folding
|Kenche H,Baty CJ,Vedagiri K,Shapiro SD,Blumental-Perry A||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (27:965)||2013|
|Non-human primate||Not Cited||
Inhibiting rotavirus infection by membrane-impermeant thiol/disulfide exchange blockers and antibodies against protein disulfide isomerase.
MA3-018 was used in blocking or activating experiment to study the ability of non-cell-penetrating thiol-disulphide exchange inhibitors to reduce rotavirus infectivity and the potential involvement of protein disulfide isomerase
|Calderon MN,Guerrero CA,Acosta O,Lopez S,Arias CF||Intervirology (55:451)||2012|
VAPB interacts with the mitochondrial protein PTPIP51 to regulate calcium homeostasis.
MA3-018 was used in western blot to investigate the effect of VAPB on calcium homeostasis modulation
|De Vos KJ,Mórotz GM,Stoica R,Tudor EL,Lau KF,Ackerley S,Warley A,Shaw CE,Miller CC||Human molecular genetics (21:1299)||2012|
Prolactin-induced changes in protein expression in human pancreatic islets.
MA3-018 was used in western blot to study prolactin-induced changes in protein expression in human pancreatic islets
|Labriola L,Ferreira GB,Montor WR,Demasi MA,Pimenta DC,Lojudice FH,Genzini T,Goldberg AC,Eliaschewitz FG,Sogayar MC||Molecular and cellular endocrinology (264:16)||2007|
Relation of the size and intracellular sorting of apoB to the formation of VLDL 1 and VLDL 2.
MA3-018 was used in western blot to compare the two-step process and an apoB size-dependent lipidation process as to the production of different lipoproteins
|Stillemark-Billton P,Beck C,Borén J,Olofsson SO||Journal of lipid research (46:104)||2005|
Regulation of calreticulin expression during induction of differentiation in human myeloid cells. Evidence for remodeling of the endoplasmic reticulum.
MA3-018 was used in western blot to investigate what affects the expression of calreticulin during the induction of differentiation of HL-60 human myeloid cells.
|Clark RA,Li SL,Pearson DW,Leidal KG,Clark JR,Denning GM,Reddick R,Krause KH,Valente AJ||The Journal of biological chemistry (277:32369)||2002|
Identification of ERp29, an endoplasmic reticulum lumenal protein, as a new member of the thyroglobulin folding complex.
MA3-018 was used in western blot to investigate the function of ERp29 in thyroid cells.
|Sargsyan E,Baryshev M,Szekely L,Sharipo A,Mkrtchian S||The Journal of biological chemistry (277:17009)||2002|
Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli.
MA3-018 was used in western blot to identify the member of CLIC5 related to the actin cytoskeleton of placental microvilli.
|Berryman M,Bretscher A||Molecular biology of the cell (11:1509)||2000|
Calreticulin, a peptide-binding chaperone of the endoplasmic reticulum, elicits tumor- and peptide-specific immunity.
MA3-018 was used in western blot to study the roles of calreticulin in tumor- and peptide-specific immunity.
|Basu S,Srivastava PK||The Journal of experimental medicine (189:797)||1999|
Role of protein disulfide isomerase and other thiol-reactive proteins in HIV-1 envelope protein-mediated fusion.
MA3-018 was used in flow cytometry and immunocytochemistry to study the role of protein disulfide isomerase and other thiol-reactive proteins in HIV-1 envelope protein-mediated fusion
|Ou W,Silver J||Virology (350:406)||2006|
Vascular expression of polycystin-2.
MA3-018 was used in immunohistochemistry to investigate the expression of polycystin-2 in vascular smooth muscle cells
|Torres VE,Cai Y,Chen X,Wu GQ,Geng L,Cleghorn KA,Johnson CM,Somlo S||Journal of the American Society of Nephrology : JASN (12:1)||2001|
PMP22 carrying the trembler or trembler-J mutation is intracellularly retained in myelinating Schwann cells.
MA3-018 was used in immunohistochemistry to study the role of PMP22 trembler or trembler-J mutations retained within the endoplasmic reticulum of myelinating Schwann cells
|Colby J,Nicholson R,Dickson KM,Orfali W,Naef R,Suter U,Snipes GJ||Neurobiology of disease (7:561)||2000|