|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||1-2 ug/ml|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant Protein corresponding to amino acids 378-525 of human PDI (ER Marker).|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
PDI (ER marker) also known as protein disulfide isomerase is an enzyme present in the ER which plays a very important role in protein folding, by facilitating formation of correct disulphide bonds. Processing and maturation of the secretory proteins in the endoplasmic reticulum are actively done by the family of PDI proteins. This class of proteins show similar homology to the cytoplasmic protein thioredoxin. The protein catalyses the thiol/disulphide interchange interactions. Also depending upon the type of polypeptide substrate and redox potential, the protein brings about protein disulfide formation, isomerization or reduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Disulphide Isomerase antibody; GIT antibody; Glutathione Insulin Transhydrogenase antibody; Pancreas-specific protein disulfide isomerase; pancreatic protein disulfide isomerase; PDIp; Procollagen Proline 2 Oxoglutarate 4 Dioxygenase beta; protein disulfide isomerase A2; protein disulfide isomerase family A, member 2; protein disulfide isomerase, pancreatic; protein disulfide isomerase-associated 2; Protein disulfide-isomerase A2; Rho GDP dissociation inhibitor gamma
Gene Aliases: PDA2; PDI; PDIA2; PDIP; PDIR
UniProt ID: (Human) Q13087
Entrez Gene ID: (Human) 64714
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.