|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
PDI (ER marker) also known as protein disulfide isomerase is an enzyme present in the ER which plays a very important role in protein folding, by facilitating formation of correct disulphide bonds. Processing and maturation of the secretory proteins in the endoplasmic reticulum are actively done by the family of PDI proteins. This class of proteins show similar homology to the cytoplasmic protein thioredoxin. The protein catalyses the thiol/disulphide interchange interactions. Also depending upon the type of polypeptide substrate and redox potential, the protein brings about protein disulfide formation, isomerization or reduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.