OriGene

PDXK Monoclonal Antibody (OTI3G2), TrueMAB™

View all (15) PDXK antibodies

Datasheet

Protocols

Questions & Answers

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Datasheet

Protocols

Questions & Answers

Tech Support

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PDXK Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-PDXK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA503031)

PDXK Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDXK (RC200975, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PDXK. Positive lysates LY418499 (100 µg) and LC418499 (20 µg) can be purchased separately from OriGene.

PDXK Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC200975 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDXK antibody (TA503031), and then analyzed by flow cytometry.

Product Details

CF503031

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:150

Flow Cytometry (Flow)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI3G2

Immunogen

Full length human recombinant protein of human PDXK produced in HEK293T cell.

View immunogen

Conjugate

Unconjugated

Form

lyophilized

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

Pyridoxal kinase (PDXK) converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA reveals pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript is expressed in all tissues tested. The expression of PDXK shows circadian oscillations. The expression of Pdxk in mouse liver and brain is regulated by the 3 PAR bZIP transcription factors, Dbp, Hlf, and Tef, which also show circadian oscillations in expression. Mice devoid of all 3 transcription factors show decreased levels of brain PLP, serotonin, and dopamine, and are highly susceptible to frequently lethal generalized spontaneous and audiogenic epilepsies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: epididymis secretory sperm binding protein Li 1a; Pyridoxal kinase; pyridoxamine kinase; Pyridoxine kinase; vitamin B6 kinase

Gene Aliases: C21orf124; C21orf97; HEL-S-1a; PDXK; PKH; PNK; PRED79

UniProt ID: (Human) O00764

Entrez Gene ID: (Human) 8566

Molecular Function: kinase

Function(s)

cell proliferation pyridoxal 5'-phosphate salvage phosphorylation vitamin B6 metabolic process pyridoxal phosphate biosynthetic process

Process(es)

magnesium ion binding ATP binding zinc ion binding pyridoxal kinase activity pyridoxal phosphate binding potassium ion binding sodium ion binding lithium ion binding protein homodimerization activity