Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis was performed on membrane enriched extracts (30 ug lysate) of HeLa (Lane 1), Hep G2 (Lane 2), SK-OV-3 (Lane 3), COS-7 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), PC-3 (Lane 7), and LNCaP (Lane 8). The blots were probed with Rabbit Anti-PEBP1 Polyclonal Antibody (Product # PA3211, 2 ug/ml) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 ug/ml, 1:4000 dilution). A 21 kDa band corresponding to PEBP1 was observed across the cell lines tested. The 42 kDa bands observed could potentially be PEBP1 dimers, caused by the PKC-mediated phosphorylation of PEBP1, as reported in literature. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal amino acids C(167)YQAEWDDYVPKLYEQLSGK(187) of PEBP1|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2000|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-211 detects PEBP1 in human, mouse, and rat samples.
PA3-211 has successfully been used in Western blot, immunocytochemical, and immunohistochemical (paraffin) procedures.
The PA3-211 immunogen is a synthetic peptide corresponding to the C-terminal amino acids C(167)YQAEWDDYVPKLYEQLSGK(187) of PEBP1
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
23 kDa morphine-binding protein; epididymis luminal protein 210; epididymis secretory protein Li 34; epididymis secretory protein Li 96; HCNPpp; hippocampal cholinergic neurostimulating peptide; Neuropolypeptide h3; P23K; PEBP-1; Phosphatidylethanolamine-binding protein 1; prostatic binding protein; Prostatic-binding protein; Raf kinase inhibitor protein; Raf kinase inhibitory protein; Raf-1 inhibitor protein; Raf-1 kinase inhibitor protein; RKIP
HCNP; HCNPpp; HEL-210; HEL-S-34; HEL-S-96; PBP; Pbp1; Pbpr; PEBP; PEBP-1; PEBP1; RKIP