Immunofluorescence analysis of Syntaxin was performed using 70% confluent log phase T-47D. The cells were fixed with Methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with ABfinity™ Syntaxin PEP12 (7H24L3) Recombinant Rabbit Monoclonal Antibody (700689) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Cytoskeleton was stained with alpha-tubulin Monoclonal Antibody (Product# 322500, 1ug/ml) followed by Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A11032, 1:400). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Protein corresponding to amino acids 1–268 of yeast Syntaxin.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2-3 µg/ml|
|Immunofluorescence (IF)||2-3 µg/ml|
|Western Blot (WB)||0.1-1 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with Yeast based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Syntaxins belong to a family of SNARE proteins. The yeast Saccharomyces cerevisiae has about 7 genes similar to syntaxin and there are presently 15 types of syntaxin genes in the human genome. In presence of other cytoplasmic proteins like the N-ethylmaleimide-sensitive factor (NSF) and SNAP; synatxins can combine various vesicular transport systems for the exocytic and endocytic pathway. This is very important for the functioning and for bringing about specificity to the vesicular combination process.. The syntaxin protein in yeast is also known as the Pep12 protein. The function of this Pep12 protein in the yeast is to transport vesicles from the Golgi apparatus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.