OriGene

PFN1 Monoclonal Antibody (OTI1D5), TrueMAB™

View all (18) Profilin 1 antibodies

Questions & Answers

Questions & Answers

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PFN1 Antibody in Immunocytochemistry (ICC/IF) — Anti-PFN1 mouse monoclonal antibody (TA501211) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PFN1(RC202338).

PFN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human pancreas tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501211, Dilution 1:50)

PFN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded human Kidney tissue within the normal limits using anti-PFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501211, Dilution 1:50)

PFN1 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PFN1 (RC202338, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PFN1. Positive lysates LY417584 (100 µg) and LC417584 (20 µg) can be purchased separately from OriGene.

PFN1 Antibody in Western Blot (WB) — Western blot analysis of extracts (10 µg) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

PFN1 Antibody in Western Blot (WB) — Western blot analysis of extracts (10 µg) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody. (1:200)

PFN1 Antibody in Western Blot (WB) — Western blot analysis of extracts (35 µg) from 9 different cell lines by using anti-PFN1 monoclonal antibody.

PFN1 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC202338 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PFN1 antibody (TA501211), and then analyzed by flow cytometry.

PFN1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Jurkat cells, using anti-PFN1 antibody (TA501211), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

PFN1 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of Hela cells, using anti-PFN1 antibody (TA501211), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

-

Immunohistochemistry (IHC)

1:50

-

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

1:100

-

Product Specifications

Species Reactivity

Dog, Human, Non-human primate, Rat

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

OTI1D5

Immunogen

Full length human recombinant protein of human PFN1 produced in HEK293T cell.

Conjugate

Unconjugated

Form

liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Target Information

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Epididymis tissue protein Li 184a; Profilin I; Profilin-1

Gene Aliases: ALS18; PFN1

UniProt ID: (Human) P07737, (Rat) P62963

Entrez Gene ID: (Dog) 607397, (Human) 5216, (Rat) 64303

Molecular Function: actin or actin-binding cytoskeletal protein cytoskeletal protein non-motor actin binding protein

neural tube closure positive regulation of epithelial cell migration actin cytoskeleton organization negative regulation of actin filament polymerization positive regulation of actin filament polymerization negative regulation of actin filament bundle assembly positive regulation of actin filament bundle assembly positive regulation of ATPase activity positive regulation of transcription from RNA polymerase II promoter positive regulation of viral transcription protein stabilization positive regulation of DNA metabolic process positive regulation of stress fiber assembly negative regulation of stress fiber assembly Wnt signaling pathway, planar cell polarity pathway cellular response to growth factor stimulus cell-cell adhesion positive regulation of ruffle assembly regulation of transcription from RNA polymerase II promoter actin polymerization or depolymerization response to organic substance

Process(es)

adenyl-nucleotide exchange factor activity actin binding actin monomer binding receptor binding protein binding phosphatidylinositol-4,5-bisphosphate binding Rho GTPase binding poly(A) RNA binding proline-rich region binding cadherin binding involved in cell-cell adhesion