Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:1,000-1:2,000 | - |
Immunohistochemistry (IHC) |
1:50 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
Flow Cytometry (Flow) |
1:100 | - |
Product Specifications | |
---|---|
Species Reactivity |
Dog, Human, Non-human primate, Rat |
Host/Isotype |
Mouse / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OTI1D5 |
Immunogen |
Full length human recombinant protein of human PFN1 produced in HEK293T cell. |
Conjugate |
Unconjugated |
Form |
liquid |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS with 1% BSA, 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role in both signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Profilin I appears to be highly expressed in most tissues except for skeletal muscle. Profilin II is predominantly expressed in brain and at lower levels in skeletal muscle, uterus and kidney. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Epididymis tissue protein Li 184a; Profilin I; Profilin-1
Gene Aliases: ALS18; PFN1
UniProt ID: (Human) P07737, (Rat) P62963
Entrez Gene ID: (Dog) 607397, (Human) 5216, (Rat) 64303
Molecular Function:
actin or actin-binding cytoskeletal protein
cytoskeletal protein
non-motor actin binding protein
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