Proteintech

PFN2 Monoclonal Antibody (2H7C12)

View all (7) Profilin 2 antibodies

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Questions & Answers

Tech Support

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PFN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PFN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

PFN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 40x lens).

PFN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using 60094-2-Ig (PFN2 Antibody) at dilution of 1:50 (under 10x lens).

PFN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 60094-2-IG ( PFN2 Antibody) at dilution of 1:400 (under 40x lens).

PFN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 60094-2-IG ( PFN2 Antibody) at dilution of 1:400 (under 10x lens).

PFN2 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-IG ( PFN2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

PFN2 Antibody in Western Blot (WB) — Rat brain tissue were subjected to SDS PAGE followed by western blot with 60094-2-Ig (PFN2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

60094-2-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

2H7C12

Immunogen

PFN2 Fusion Protein Ag5043 (1-140 aa encoded by BC018049)

Conjugate

Unconjugated

Form

Liquid

Concentration

1.5 mg/mL

Purification

Caprylic acid precipitation

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Product Specific Information

The MW of this protein is 15 kDa, and this antibody specifically recognizes the 15 kDa protein.

Immunogen sequence: MAGWQSYVD NLMCDGCCQE AAIVGYCDAK YVWAATAGGV FQSITPIEID MIVGKDREGF FTNGLALGAK KCSVIRDSLY VDGDCTMDIR TKSQGGEPTY NVAVGRAGRV LVFVMGKEGV HGGGLNKKAY SMAKYLRDSG F (1-140 aa encoded by BC018049)

Target Information

Profilin 2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Circ_0008500 Knockdown Improves Radiosensitivity and Inhibits Tumorigenesis in Breast Cancer Through the miR-758-3p/PFN2 Axis.

Journal of mammary gland biology and neoplasia

Kong D,Shen D,Liu Z,Zhang J,Zhang J,Geng C

Tue Mar 01 00:00:00 EST 2022

Circ_0008500 Knockdown Improves Radiosensitivity and Inhibits Tumorigenesis in Breast Cancer Through the miR-758-3p/PFN2 Axis.

Journal of mammary gland biology and neoplasia

Kong D,Shen D,Liu Z,Zhang J,Zhang J,Geng C

Tue Mar 01 00:00:00 EST 2022

ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy.

Molecular medicine (Cambridge, Mass.)

Lu L,Zhong Z,Gu J,Nan K,Zhu M,Miao C

Thu Jul 08 00:00:00 EDT 2021

ets1 associates with KMT5A to participate in high glucose-mediated EndMT via upregulation of PFN2 expression in diabetic nephropathy.

Molecular medicine (Cambridge, Mass.)

Lu L,Zhong Z,Gu J,Nan K,Zhu M,Miao C

Thu Jul 08 00:00:00 EDT 2021

NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis.

The Journal of cell biology

Huang R,Yuan DJ,Li S,Liang XS,Gao Y,Lan XY,Qin HM,Ma YF,Xu GY,Schachner M,Sytnyk V,Boltze J,Ma QH,Li S

Mon Jan 06 00:00:00 EST 2020

Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer.

International journal of oncology

Zhang H,Yang W,Yan J,Zhou K,Wan B,Shi P,Chen Y,He S,Li D

Sat Sep 01 00:00:00 EDT 2018

Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer.

International journal of oncology

Zhang H,Yang W,Yan J,Zhou K,Wan B,Shi P,Chen Y,He S,Li D

Sat Sep 01 00:00:00 EDT 2018

Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer.

International journal of oncology

Zhang H,Yang W,Yan J,Zhou K,Wan B,Shi P,Chen Y,He S,Li D

Sat Sep 01 00:00:00 EDT 2018

Loss of profilin 2 contributes to enhanced epithelial-mesenchymal transition and metastasis of colorectal cancer.

International journal of oncology

Zhang H,Yang W,Yan J,Zhou K,Wan B,Shi P,Chen Y,He S,Li D

Sat Sep 01 00:00:00 EDT 2018

PFN2a, a new partner of RARα in the cytoplasm.

Biochemical and biophysical research communications

Andriamoratsiresy D,Piskunov A,Lutzing R,Rochette-Egly C

Mon Jan 01 00:00:00 EST 2018

PFN2a, a new partner of RARα in the cytoplasm.

Biochemical and biophysical research communications

Andriamoratsiresy D,Piskunov A,Lutzing R,Rochette-Egly C

Mon Jan 01 00:00:00 EST 2018

PFN2a, a new partner of RARα in the cytoplasm.

Biochemical and biophysical research communications

Andriamoratsiresy D,Piskunov A,Lutzing R,Rochette-Egly C

Mon Jan 01 00:00:00 EST 2018

PFN2a, a new partner of RARα in the cytoplasm.

Biochemical and biophysical research communications

Andriamoratsiresy D,Piskunov A,Lutzing R,Rochette-Egly C

Mon Jan 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Profilin II; Profilin-2

Gene Aliases: D3S1319E; PFL; Pfn; PFN2

UniProt ID: (Human) P35080, (Mouse) Q9JJV2, (Rat) Q9EPC6

Entrez Gene ID: (Human) 5217, (Pig) 100621523, (Mouse) 18645, (Rat) 81531

Molecular Function: non-motor actin binding protein

negative regulation of epithelial cell migration actin cytoskeleton organization negative regulation of actin filament polymerization positive regulation of actin filament polymerization positive regulation of actin filament bundle assembly positive regulation of ATPase activity positive regulation of peptidyl-serine phosphorylation protein stabilization positive regulation of stress fiber assembly negative regulation of ruffle assembly regulation of synaptic vesicle exocytosis regulation of actin filament polymerization

Process(es)

adenyl-nucleotide exchange factor activity actin monomer binding protein binding phosphatidylinositol-4,5-bisphosphate binding actin binding