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PHF11 Antibody (MA5-55520) in WB
Western blot analysis of lysates from wild type (WT) and PHF11 knockdown (KD) 293T cells, using PHF11 Recombinant Monoclonal Antibody (Product # MA5-55520) at 1:2,000 dilution followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) secondary antibody at 1:10,000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-55520
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1J3E4
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 40-321 of human PHF11.
Conjugate
Form
Liquid
Concentration
1.77 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
RRID
AB_3667759
Product Specific Information
Immunogen Sequence: MEKRTCALCP KDVEYNVLYF AQSENIAAHE NCLLYSSGLV ECEDQDPLNP DRSFDVESVK KEIQRGRKLK CKFCHKRGAT VGCDLKNCNK NYHFFCAKKD DAVPQSDGVR GIYKLLCQQH AQFPIIAQSA KFSGVKRKRG RKKPLSGNHV QPPETMKCNT FIRQVKEEHG RHTDATVKVP FLKKCKEAGL LNYLLEEILD KVHSIPEKLM DETTSESDYE EIGSALFDCR LFEDTFVNFQ AAIEKKIHAS QQRWQQLKEE IELLQDLKQT LCSFQENRDL MS
Target Information
PHF11 contains two PHD zinc fingers and probably regulates transcription. Distinctive splice variants were expressed in immune tissues and cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: atopic; BRCA1 C-terminus-associated protein; IgE responsiveness; IgE responsiveness (atopic); NY-REN-34 antigen; PHD finger protein 11; PHD finger protein 11 family member; PHD finger protein 11-like; PHD finger protein 11A; PHD finger protein 11D; Renal carcinoma antigen NY-REN-34; RP11-185C18.3
Gene Aliases: 4933417L10Rik; APY; BCAP; D14Ertd668e; IGEL; IGER; IGHER; NY-REN-34; NYREN34; PHF11; Phf11-4; Phf11a; Phf11d; Phf11l
UniProt ID: (Human) Q9UIL8, (Rat) Q5I0E2, (Mouse) Q8BVM9, (Mouse) A6H5X4
Entrez Gene ID: (Human) 51131, (Rat) 361051, (Mouse) 219131, (Mouse) 219132
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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