|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Pig, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified fragment (aa 333 - 430) of recombinant human PI-3-K p85 alpha subunit.|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21830 detects PI 3 Kinase p85 alpha in Human, Rat and Mouse samples.
MA1-21830 has been successfully used in Immunofluorescence and Western Blot procedures. By Western Blot this antibody detects a band of 85kDa, which corresponds to the predicted molecular weight of PI 3 kinase.
MA1-21830 immunogen corresponds to Purified fragment (aa 333 - 430) of recombinant human PI-3-K p85 alpha subunit..
The enzyme PI-3-K phosphorylates at the D-3 position of the inositol ring of PI, PI-4- phosphate, and PI-4,5-biphosphate (PI-4,5-P2) to produce their respective PI3-phosphorylated derivatives. PI-3-K exists as a heterodimer of 85 kDa (p85) and 110 kDa (p110) subunits. The p85 subunit contains two SH2 domains and an SH3 domain. It has been shown to associate with and serve as a substrate for activated growth factor receptor tyrosine kinases. p85 may serve as regulator of the catalytic subunit, p110, by acting as the link between PI-3-K and the ligand-activated receptor. Two distinct forms of the p85 subunit are described: p85 alpha, which binds tightly to the catalytic subunit and p85 beta, a protein of unknown function. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phosphatidylinositol 4,5-bisphosphate mediates Ca2+-induced platelet alpha-granule secretion: evidence for type II phosphatidylinositol 5-phosphate 4-kinase function.
MA1-21830 was used in flow cytometry to study the role of phosphatidylinositol 4,5-bisphosphate in platelet granule release
|Rozenvayn N,Flaumenhaft R||The Journal of biological chemistry (276:22410)||2001|
A repressor sequence in the juxtamembrane domain of Flt-1 (VEGFR-1) constitutively inhibits vascular endothelial growth factor-dependent phosphatidylinositol 3'-kinase activation and endothelial cell migration.
MA1-21830 was used in western blot to identify which domains are responsible for the functional differences between VEGFR-1 and VEGFR-2
|Gille H,Kowalski J,Yu L,Chen H,Pisabarro MT,Davis-Smyth T,Ferrara N||The EMBO journal (19:4064)||2000|