|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Baculovirus expressed (recombinant) Bovine p85 beta subunit of PI 3 Kinase.|
|Storage buffer||tissue culture supernatant diluted in 0.2M tris HCl, pH 7.4, with 5-10% FBS|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:50-1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-21473 detects PI3 Kinase p85 beta in human and monkey samples.
MA1-21473 has been successfully used in immunohistochemistry (frozen), immunoprecipitation and Western blot procedures.
MA1-21473 immunogen corresponds to Baculovirus expressed (recombinant) Bovine p85 beta subunit of PI 3 Kinase.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.