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PIKFYVE Antibody (CF810373) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIKFYVE (RC230258, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-PIKFYVE. (1:2000)
Product Details
CF810373
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
OTI1D3
Immunogen
Full length human recombinant protein of human PIKFYVE produced in E.coli.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
PIKFYVE belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. The content of phosphatidylinositol 3,5-bisphosphateP2) in endosomal membranes changes dynamically with fission and fusion events that generate or absorb intracellular transport vesicles. PIKFYVE is the PtdIns(3,5)P2-producing component of a trimolecular complex that tightly regulates the level of PtdIns(3,5)P2. Other components of this complex are the PIKFYVE activator VAC14 and the PtdIns(3,5)P2 phosphatase FIG4. In addition to its phosphoinositide 5-kinase activity, PIKFYVE also has protein kinase activity. For a review, see Shisheva.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1-phosphatidylinositol 3-phosphate 5-kinase; 1-phosphatidylinositol-3-phosphate 5-kinase; epididymis luminal protein 37; FYVE finger-containing phosphoinositide kinase; p235; Phosphatidylinositol 3-phosphate 5-kinase; Phosphatidylinositol 3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate 5-kinase type III; phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III; phosphatidylinositol-4-phosphate 5-kinase, type III; phosphoinositide kinase, FYVE finger containing; PIKfyve; PIPkin-III; PtdIns(4)P-5-kinase; Serine-protein kinase PIKFYVE; type III PIP kinase; zinc finger, FYVE domain containing 29
Gene Aliases: 5230400C17Rik; CFD; FAB1; HEL37; KIAA0981; p235; PIKFYVE; PIP5K; PIP5K3; Pipk5k3; PipkIII; ZFYVE29
UniProt ID: (Human) Q9Y2I7, (Mouse) Q9Z1T6
Entrez Gene ID: (Human) 200576, (Rat) 316457, (Mouse) 18711
1-phosphatidylinositol-3-phosphate 5-kinase activity
protein binding
ATP binding
zinc ion binding
1-phosphatidylinositol-4-phosphate 5-kinase activity
phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity
phosphatidylinositol phosphate kinase activity
metal ion binding
nucleotide binding
kinase activity
transferase activity
kinase
transferase
metabolite interconversion enzyme
phosphatidylinositol biosynthetic process
receptor-mediated endocytosis
myelin assembly
protein localization to nucleus
intracellular signal transduction
phosphatidylinositol-3-phosphate biosynthetic process
retrograde transport, endosome to Golgi
phosphatidylinositol phosphorylation
phosphatidylinositol 5-phosphate metabolic process
regulation of autophagosome assembly
cellular protein metabolic process
phosphorylation
phosphatidylinositol metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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