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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from near C-terminus of human PIM2 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is B cell lymphoma.
PIM-2 belongs to a family of cytoplasmic serine/threonine protein kinases that consists of two other members, PIM-1 and PIM-3. Expression of this protein is regulated by hematopoietic cytokines. PIM-2 is most abundantly expressed in hematopoietic tissues, spleen, thymus, and peripheral blood leukocytes, as well as in testis, small intestine, and colon. It is also highly expressed in human leukemic and lymphoma cell lines. The gene is also expressed in primary spermatocytes. The results suggest a role for PIM-2 in proliferating cells as well as during meiosis. PIM-2 is thought to be a mediator of p53 mediated apoptosis inhibition by interleukin 3, which supports its role in the formation of various tumors including lymphomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
pim-2 oncogene; pim-2h; proto-oncogene Pim-2 (serine threonine kinase); proto-oncogene Pim-2 serine threonine kinase; serine/threonine protein kinase pim-2; serine/threonine-protein kinase pim-2