|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acids 90–213 of human Hiwi|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
This antibody is predicted to react with mouse based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Hiwi is a member of the Piwi /Argonaute protein family. Hiwi is specifically expressed in germline cells such as spermatocytes and round spermatids during spermatogenesis. Hiwi is also expressed in CD34+ hematopoietic progenitors, but not in CD34– hematopoietic cells, suggesting a role for this gene in the maintenance of hematopoietic stem cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Manipulations in HIWI level exerts influence on the proliferation of human non-small cell lung cancer cells.
701177 was used in western blot to determine the exterted influence on the proliferation of human non-small cell lung cancer cells by manipulations in HIWI levels
|Wang Y,Liu J,Wu G,Yang F||Experimental and therapeutic medicine (11:1971)||2016|