|Tested species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human protein purified from E.coli (GST-mili-1)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is mouse testis.
The piwi gene of Drosophila belongs to a novel class of evolutionarily conserved genes (the piwi or Argonaute family). The Piwi family and quote; is required for germ-and stem-cell development in invertebrates, and two Piwi members, MIWI and MILI (Miwi like; Piwil2; Piwi-like protein 2), are essential for spermatogenesis in mouse. MIWI and MILI partner with Piwi-interacting RNAs (piRNAs) which are 26-31 nucleotides (nt) in length—clearly distinct from the 21-23 nt of microRNAs (miRNAs) or short interfering RNAs (siRNAs). A search for murine small RNAs that might program Piwi proteins for transposon suppression revealed developmentally regulated piRNA loci, some of which resemble transposon master control loci of Drosophila. MILI might be programmed by distinct piRNA populations at different stages of germ cell development, suggesting MILI plays an important role in meiotic prophase progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.