|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Clone||H2 006 D4|
|Immunogen||Column purified fraction of cAMP dependent PKA RII from human saliva.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:100|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA3-517 detects PKA RII protein in human samples. MA3-517 does not discriminate between RII alpha and beta.
MA3-517 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~50 kDa protein representing PKA RII in human saliva.
MA3-517 has successfully been used in ChIP analysis of PKARII.
The MA3-517 immunogen is a purified fraction of cAMP dependent PKA RII from human saliva.
The type 1(RI) and type 2 (RII) subunit of cyclic AMP-dependent protein kinase A (PKA) is a polyfunctional protein that is present in most tissues and cells. The many roles of RII include: 1) the regulation of extracellular cyclic AMP levels, 2) catalyzing the phosphorylation of the transcription factor CREB, 3) aiding in the development of mammalian oocytes and 4) phosphorylation of proteins to enhance the release of neurotransmitters. Localization of PKA is coordinated through association with A-kinase anchoring proteins (AKAPs) that bind to a 14-18aa sequence in subunit RII.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Adenylyl cyclase 3/adenylyl cyclase-associated protein 1 (CAP1) complex mediates the anti-migratory effect of forskolin in pancreatic cancer cells.
MA3-517 was used in western blot to study the expression of transmembrane AC isoforms in pancreatic cancer cells
|Quinn SN,Graves SH,Dains-McGahee C,Friedman EM,Hassan H,Witkowski P,Sabbatini ME||Molecular carcinogenesis (56:1344)||2017|