Invitrogen

PKC beta-2 Recombinant Rabbit Monoclonal Antibody (ST48-06)

View all (4) PKC beta-2 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

PKC beta-2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of PKC beta-1 in SH-SY-5Y cells using a PKC beta-1 Monoclonal antibody (Product # MA5-32161) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

PKC beta-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of PKC beta-1 of paraffin-embedded Mouse spleen tissue using a PKC-beta-1 Monoclonal antibody (Product #MA5-32161). Counter stained with hematoxylin.

PKC beta-2 Antibody in Western Blot (WB) — Western blot was performed using Anti-PKC beta-2 Monoclonal Antibody (Product # MA5-32161) and a 78 kDa band corresponding to PKC beta-2 was observed across the cell lines and tissues tested. Whole cell extracts (30ug) of K-562 (Lane 1), Jurkat (Lane 2), MOLT4 (Lane 3), Mouse Brain (Lane 4) and Mouse Liver (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

PKC beta-2 Antibody in Flow Cytometry (Flow) — Flow Cytometric analysis of PKC beta-1 in Hela cells using a PKC beta-1 Monoclonal Antibody (Product # MA5-32161) at a dilution of 1:50, as seen in blue compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Product Details

MA5-32161

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

Immunohistochemistry (IHC)

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

ST48-06

Immunogen

Synthetic peptide within Human PKC beta 2 aa 615-660

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2809450

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The PKC family of serine/threonine kinases, including PRKCB2 (PKC beta II), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: PKC-B; PKC-beta; protein kinase C beta I; protein kinase C beta II; Protein kinase C beta type; protein kinase C beta-II; protein kinase C, beta 1; protein kinase C, beta 1 polypeptide

Gene Aliases: A130082F03Rik; PKC-Beta; PKCB; PRKCB; PRKCB1; PRKCB2

UniProt ID: (Human) P05771, (Mouse) P68404, (Rat) P68403

Entrez Gene ID: (Human) 5579, (Mouse) 18751, (Rat) 25023

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme