Invitrogen

PKC epsilon Monoclonal Antibody (H.51.9)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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PKC epsilon Antibody in Western Blot (WB)

FIGURE: 1 / 5

PKC epsilon Antibody (MA5-14908) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), HCT 116 (Lane 2), HT-29 (Lane 3), A549 (Lane 4), PC-3 (Lane 5), PC-12 (Lane 6), SH-SY5Y (Lane 7), Neuro-2a (Lane 8) and tissue extract of Mouse Brain (Lane 9). The blot was probed with anti-PKC epsilon Antibody (Product # MA5-14908, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 84 kDa band corresponding to PKC... View More

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PKC epsilon Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), HCT 116 (Lane 2), HT-29 (Lane 3), A549 (Lane 4), PC-3 (Lane 5), PC-12 (Lane 6), SH-SY5Y (Lane 7), Neuro-2a (Lane 8) and tissue extract of Mouse Brain (Lane 9). The blot was probed with anti-PKC epsilon Antibody (Product # MA5-14908, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 84 kDa band corresponding to PKC epsilon was observed across all the cell lines and tissue tested.

PKC epsilon Antibody in Western Blot (WB) — Western blot analysis of PKC Epsilon in extracts from mouse brain and PC12 cells using PKC- Epsilon monoclonal antibody (Product # MA5-14908).

PKC epsilon Antibody in Western Blot (WB) — Knockdown of PKC epsilon was achieved by transfecting A549 with PKC epsilon specific siRNAs (Silencer® select Product # s11102, s11101). Western blot analysis (Fig. a) was performed using whole cell extracts from the PKC epsilon knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PKC epsilon Monoclonal Antibody (H.51.9) (Product # MA5-14908, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Absence of signal upon siRNA mediated knock down confirms that antibody is specific to PKC epsilon.

PKC epsilon Antibody in Western Blot (WB) — Translocation of PKCepsilon (protein kinase C epsilon) to the particulate fraction with preconditioning. Western blot analysis was used to measure PKCepsilon translocation to the mitochondria-enriched particulate fraction ( A ). Densitometric comparison of PKCepsilon signal between cytosolic and particulate fractions revealed the translocation of PKCepsilon to the mitochondria-enriched particulate fraction in the ischemic preconditioning (IPC) animals compared with no IPC controls ( B ). Mitochondria enrichment in the particulate fraction was measured using an antibody toward the beta subunit of complex V adenosine triphosphate synthase beta (ATPbeta), showing equal enrichment in mitochondria in both no IPC and IPC samples ( C ). C indicates cytosolic fraction; DB71, direct blue 71 staining, a general protein staining to control for equal loading and transfer efficiency; and P, particulate fraction. Cyt indicates the term cytosolic fraction and Part indicates particulate fraction. An asterisk (*) indicates statistically signifant difference and more the asterisk count more statistical difference.

PKC epsilon Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with PKC epsilon siRNA and absence of signal was observed in Western Blot using Anti-PKC epsilon Monoclonal Antibody (H.51.9) (Product # MA5-14908). {KD}

Product Details

MA5-14908

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

H.51.9

Immunogen

Synthetic peptide corresponding to human PKCe

Conjugate

Unconjugated

Form

Liquid

Concentration

405 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10985232

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other PKC isoforms.

Target Information

The PKC family of serine/threonine kinases, including PRKCE (PKC epsilon), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pulseless Electrical Activity as the Initial Cardiac Arrest Rhythm: Importance of Preexisting Left Ventricular Function.

Journal of the American Heart Association

Ambinder DI,Patil KD,Kadioglu H,Wetstein PS,Tunin RS,Fink SJ,Tao S,Agnetti G,Halperin HR

Published figure using PKC epsilon monoclonal antibody (Product # MA5-14908) in Western Blot

Tue Jul 06 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: MGC125656; MGC125657; nPKC-epsilon; PKC e; Protein kinase C epsilon type; protein kinase C, epsilon

Gene Aliases: 5830406C15Rik; nPKC-epsilon; PKC[e]; PKCE; Pkcea; PKCepsilon; PRKCE; R75156

UniProt ID: (Human) Q02156, (Mouse) P16054, (Rat) P09216

Entrez Gene ID: (Human) 5581, (Mouse) 18754, (Rat) 29340

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

macrophage activation involved in immune response protein phosphorylation apoptotic process cell cycle cell adhesion signal transduction activation of phospholipase C activity positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion peptidyl-serine phosphorylation platelet activation positive regulation of actin filament polymerization negative regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis intracellular signal transduction locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of I-kappaB kinase/NF-kappaB signaling response to morphine positive regulation of MAPK cascade regulation of peptidyl-tyrosine phosphorylation positive regulation of lipid catabolic process release of sequestered calcium ion into cytosol regulation of release of sequestered calcium ion into cytosol cell division regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing positive regulation of receptor activity negative regulation of sodium ion transmembrane transporter activity positive regulation of cellular glucuronidation immune system process chemosensory behavior negative regulation of mitochondrial membrane potential phosphorylation regulation of lipid metabolic process cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process positive regulation of catalytic activity negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration

Process(es)

actin monomer binding protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity signal transducer activity protein binding ATP binding enzyme activator activity enzyme binding receptor activator activity ethanol binding metal ion binding 14-3-3 protein binding nucleotide binding protein kinase activity receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups SH3 domain binding protein kinase binding