Invitrogen

PKC epsilon Monoclonal Antibody (H.51.9)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (49) PKC epsilon antibodies

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Questions & Answers

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PKC epsilon Antibody in Western Blot (WB) — Knockdown of PKC epsilon was achieved by transfecting A549 with PKC epsilon specific siRNAs (Silencer® select Product # s11102, s11101). Western blot analysis (Fig. a) was performed using whole cell extracts from the PKC epsilon knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PKC epsilon Monoclonal Antibody (H.51.9) (Product # MA5-14908, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Absence of signal upon siRNA mediated knock down confirms that antibody is specific to PKC epsilon.

PKC epsilon Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), HCT 116 (Lane 2), HT-29 (Lane 3), A549 (Lane 4), PC-3 (Lane 5), PC-12 (Lane 6), SH-SY5Y (Lane 7), Neuro-2a (Lane 8) and tissue extract of Mouse Brain (Lane 9). The blot was probed with anti-PKC epsilon Antibody (Product # MA5-14908, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 84 kDa band corresponding to PKC epsilon was observed across all the cell lines and tissue tested.

PKC epsilon Antibody in Western Blot (WB) — Western blot analysis of PKC Epsilon in extracts from mouse brain and PC12 cells using PKC- Epsilon monoclonal antibody (Product # MA5-14908).

PKC epsilon Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with PKC epsilon siRNA and absence of signal was observed in Western Blot using Anti-PKC epsilon Monoclonal Antibody (H.51.9) (Product # MA5-14908). {KD}

Product Details

MA5-14908

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	-

Product Specifications
Species Reactivity	Chicken, Human, Mouse, Non-human primate, Rat
Host/Isotype	Rabbit / IgG
Class	Monoclonal
Type	Antibody
Clone	H.51.9
Immunogen	Synthetic peptide corresponding to human PKCe
Conjugate	Unconjugated
Form	Liquid
Purification	Affinity chromatography
Storage buffer	0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains	<0.02% sodium azide
Storage conditions	-20°C
RRID	AB_10985232

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other PKC isoforms.

Target Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: MGC125656; MGC125657; nPKC-epsilon; PKC e; Protein kinase C epsilon type; protein kinase C, epsilon

Gene Aliases: 5830406C15Rik; nPKC-epsilon; PKC[e]; PKCE; Pkcea; PKCepsilon; PRKCE; R75156

UniProt ID: (Human) Q02156, (Mouse) P16054, (Rat) P09216

Entrez Gene ID: (Human) 5581, (Chicken) 421409, (Mouse) 18754, (Rat) 29340

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

macrophage activation involved in immune response protein phosphorylation apoptotic process cell cycle cell adhesion signal transduction activation of phospholipase C activity positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion peptidyl-serine phosphorylation platelet activation positive regulation of actin filament polymerization negative regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis intracellular signal transduction locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of I-kappaB kinase/NF-kappaB signaling response to morphine positive regulation of MAPK cascade regulation of peptidyl-tyrosine phosphorylation positive regulation of lipid catabolic process release of sequestered calcium ion into cytosol regulation of release of sequestered calcium ion into cytosol cell division regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing positive regulation of receptor activity negative regulation of sodium ion transmembrane transporter activity positive regulation of cellular glucuronidation immune system process chemosensory behavior negative regulation of mitochondrial membrane potential phosphorylation regulation of lipid metabolic process cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process positive regulation of catalytic activity negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration

Process(es)

actin monomer binding protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity signal transducer activity protein binding ATP binding enzyme activator activity enzyme binding receptor activator activity ethanol binding metal ion binding 14-3-3 protein binding nucleotide binding protein kinase activity receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups SH3 domain binding protein kinase binding