Invitrogen

PKC epsilon Monoclonal Antibody (H.51.9)

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This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details

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PKC epsilon Antibody in Western Blot (WB) — Western blot analysis of PKC Epsilon in extracts from mouse brain and PC12 cells using PKC- Epsilon monoclonal antibody (Product # MA5-14908).

PKC epsilon Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), HCT 116 (Lane 2), HT-29 (Lane 3), A549 (Lane 4), PC-3 (Lane 5), PC-12 (Lane 6), SH-SY5Y (Lane 7), Neuro-2a (Lane 8) and tissue extract of Mouse Brain (Lane 9). The blot was probed with Anti- PKC epsilon Antibody (Product # MA5-14908, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/mL, 1:4000 dilution). A 84 kDa band corresponding to PKC epsilon was observed across all the cell lines and tissue tested.

PKC epsilon Antibody in Western Blot (WB) — Knockdown of PKC epsilon was achieved by transfecting A549 with PKC epsilon specific siRNAs (Silencer® select Product # s11102, s11101). Western blot analysis (Fig. a) was performed using whole cell extracts from the PKC epsilon knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PKC epsilon Monoclonal Antibody (H.51.9) (Product # MA5-14908, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Absence of signal upon siRNA mediated knock down confirms that antibody is specific to PKC epsilon.

PKC epsilon Antibody in KnockDown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with PKC epsilon siRNA and absence of signal was observed in Western Blot using Anti-PKC epsilon Monoclonal Antibody (H.51.9) (Product # MA5-14908). {KD}

Product Details

Tested Applications

Dilution

Western Blot (WB)

1:1000

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Non-human primate, Rat

Host / Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

H.51.9

Immunogen

Synthetic peptide corresponding to human PKCe

Conjugate

Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10985232

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other PKC isoforms.

Target Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: nPKC-epsilon; Protein kinase C epsilon type; protein kinase C, epsilon

Gene Aliases: 5830406C15Rik; nPKC-epsilon; PKC[e]; PKCE; Pkcea; PKCepsilon; PRKCE; R75156

UniProt ID: (Human) Q02156, (Mouse) P16054, (Rat) P09216

Entrez Gene ID: (Human) 5581, (Mouse) 18754, (Rat) 29340

Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase

macrophage activation involved in immune response protein phosphorylation apoptotic process cell cycle cell adhesion signal transduction activation of phospholipase C activity positive regulation of epithelial cell migration positive regulation of fibroblast migration positive regulation of cell-substrate adhesion peptidyl-serine phosphorylation platelet activation positive regulation of actin filament polymerization negative regulation of protein ubiquitination lipopolysaccharide-mediated signaling pathway positive regulation of insulin secretion positive regulation of synaptic transmission, GABAergic positive regulation of cytokinesis intracellular signal transduction locomotory exploration behavior TRAM-dependent toll-like receptor 4 signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis positive regulation of I-kappaB kinase/NF-kappaB signaling response to morphine positive regulation of MAPK cascade regulation of peptidyl-tyrosine phosphorylation positive regulation of lipid catabolic process release of sequestered calcium ion into cytosol regulation of release of sequestered calcium ion into cytosol cell division regulation of insulin secretion involved in cellular response to glucose stimulus positive regulation of mucus secretion cellular response to ethanol cellular response to prostaglandin E stimulus cellular response to hypoxia positive regulation of wound healing positive regulation of receptor activity negative regulation of sodium ion transmembrane transporter activity positive regulation of cellular glucuronidation immune system process chemosensory behavior negative regulation of mitochondrial membrane potential phosphorylation regulation of lipid metabolic process cellular response to platelet-derived growth factor stimulus negative regulation of apoptotic process positive regulation of catalytic activity negative regulation of release of sequestered calcium ion into cytosol negative regulation of mitochondrial calcium ion concentration

Process(es)

actin monomer binding protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity signal transducer activity protein binding ATP binding enzyme activator activity enzyme binding receptor activator activity ethanol binding metal ion binding 14-3-3 protein binding nucleotide binding protein kinase activity receptor binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups SH3 domain binding protein kinase binding

Suggested Secondary Antibodies