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Invitrogen
PKC eta Recombinant Rabbit Monoclonal Antibody (JE32-41)
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FIGURE: 1 / 6
PKC eta Antibody (MA562411) in ICC/IF
Immunofluorescence analysis of PKC eta/PRKCH was performed using 70% confluent log phase BeWo cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PKC eta Recombinant Rabbit Monoclonal Antibody (JE32-41) (Product # MA562411) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2,000 dilutio... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA562411
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE32-41
Immunogen
Recombinant protein within Mouse PKC eta aa 584-683/683.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: MCF7 cell lysate, A431 cell lysate, COS-1 cell lysate, mouse brain tissue lysate, rat brain tissue lysate.
Tissue Specificity: Low tissue specificity.
Subcellular Location: Cytoplasm.
Sequence Similarities: 97% Mouse/Rat.
Predicted band size: 78 kDa.
Target Information
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC26269; MGC5363; nPKC-eta; PKC-L; Protein uPEP2; unnamed protein product
Gene Aliases: nPKC-eta; PKC-L; Pkch; PKCL; PRKCL; uORF2
UniProt ID: (Human) P24723, (Mouse) P23298, (Rat) Q64617
Entrez Gene ID: (Human) 5583, (Mouse) 18755, (Rat) 81749
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
calcium-independent protein kinase C activity
protein binding
ATP binding
enzyme binding
protein serine/threonine kinase inhibitor activity
small GTPase binding
translation regulator activity
protein serine kinase activity
protein serine/threonine kinase binding
protein phosphorylation
signal transduction
positive regulation of macrophage derived foam cell differentiation
negative regulation of translation
positive regulation of translation in response to stress
cellular response to amino acid starvation
negative regulation of glial cell apoptotic process
intracellular signal transduction
positive regulation of keratinocyte differentiation
positive regulation of B cell receptor signaling pathway
positive regulation of glial cell proliferation
phospholipase C/protein kinase C signal transduction
positive regulation of protein localization to plasma membrane
regulation of bicellular tight junction assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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