|Tested species reactivity||Human, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified, full-length native human protein kinase D2|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-23446 detects Protein Kinase D2 (PKD2) in human samples. According to customer data, this antibody can detect pure human PKD2, endogenous PKD2 in resting neonatal rat cardiac fibroblasts, and it reacts weakly in resting neonatal rat cadiomyocytes. However, it does not detect PKD2 in PMA stimulated rat cells or pure phosphorylated PKD2.
PA1-23446 has been successfully used in ELISA and Western blot procedures.
PA1-23446 immunogen is purified, full-length native human protein kinase D2.
Protein kinase D2 (PKD2) is a novel human serine threonine protein kinase gene. It exhibits the strongest homology to the serine threonine protein kinases PKD/PKC and mgr; and PKCnu, particularly in the duplex zinc finger-like cysteine-rich motif, in the pleckstrin homology domain and in the protein kinase domain. In contrast, it shows only a low degree of sequence similarity to other members of the PKC family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein kinase D isoforms are activated in an agonist-specific manner in cardiomyocytes.
PA1-23446 was used in western blot to investigate the regulation of protein kinase D modification in heart cells
|Guo J,Gertsberg Z,Ozgen N,Sabri A,Steinberg SF||The Journal of biological chemistry (286:6500)||2011|