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PKM Antibody (MA5-38278) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of PKM1/PKM2 in A549 cells using PKM Monoclonal Antibody (Product # MA5-38278) diluted at 1:230, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
Product Details
MA5-38278
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
6C3C7
Immunogen
Recombinant Human Pyruvate kinase PKM protein (2-531aa)
Conjugate
Form
Liquid
Concentration
4.66 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2898193
Product Specific Information
This antibody has been tested in direct-ELISA
Target Information
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP:pyruvate 2-o-phosphotransferase; CTHBP; Cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; m2pk; OIP-3; OPA-interacting protein 3; p58; PK, muscle type; Pyruvate kinase 2/3; pyruvate kinase 3; Pyruvate kinase isozymes M1/M2; Pyruvate kinase muscle isozyme; Pyruvate kinase PKM; pyruvate kinase, muscle; THBP1; Threonine-protein kinase PKM2; Thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; Tumor M2-PK; Tyrosine-protein kinase PKM2
Gene Aliases: AA414905; AL024370; AL024424; CTHBP; HEL-S-30; OIP3; Pk-2; Pk-3; PK2; PK3; PKM; PKM12; PKM2; Pykm; TCB; THBP1
UniProt ID: (Rabbit) P11974, (Human) P14618, (Mouse) P52480, (Rat) P11980
Entrez Gene ID: (Rabbit) 100008676, (Human) 5315, (Mouse) 18746, (Rat) 25630
magnesium ion binding
pyruvate kinase activity
protein binding
ATP binding
kinase activity
MHC class II protein complex binding
potassium ion binding
ADP binding
poly(A) RNA binding
cadherin binding involved in cell-cell adhesion
nucleotide binding
catalytic activity
transferase activity
metal ion binding
kinase
response to hypoxia
liver development
ATP biosynthetic process
response to nutrient
response to gravity
programmed cell death
response to muscle inactivity
organ regeneration
response to insulin
pyruvate biosynthetic process
skeletal muscle tissue regeneration
canonical glycolysis
cell-cell adhesion
glucose metabolic process
glycolytic process
metabolic process
phosphorylation
response to organic substance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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