|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the internal region of human PKNOX2|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 50% glycerol, 150mM NaCl|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PREP-2 (Pbx-regulating protein-2), also known as PBX/knotted 1 homeobox 2 or PKNOX2, is a widely expressed protein belonging to the TALE (three amino acid loop extension)/MEIS family. PREP-2 is a DNA-binding protein that forms stable complexes with Pbx proteins. It is highly homologous to the related protein PREP-1, but displays a more restricted tissue distribution and a higher DNA-dissociation rate. Like PREP-1, PREP-2 forms a heterodimer with Pbx 1. The PREP-2-Pbx 1 dimer is relocated to the nucleus where it associates with HoxB1 to form a ternary complex. In contrast with PREP-1, which acts to increase transcriptional activation in this ternary complex, PREP-2 leads to a slight decrease in transcriptional activity of the ternary complex. Multiple isoforms exist for PREP-2, localizing to the nucleus or cytoplasm. Cytoplasmic isoforms are believed to colocalize with F-actin, G-actin and tubulin/microtubules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.