|Immunocytochemistry (ICC)||5 ug/ml|
|Immunofluorescence (IF)||5 ug/ml|
|Western Blot (WB)||1-3 µg/mL|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A recombinant protein corresponding to amino acids 54-158.|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
This antibody is predicted to react with Rhesus monkey based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Double-stranded RNA-dependent protein kinase (PKR) is a 68 kDa protein that is induced by interferon and double-stranded RNAs (dsRNA) produced in virus-infected cells. Two dsRNA-binding domains in the N-terminus interact with dsRNA to modify the conformation of PKR and allow it to undergo autophosphorylation and activation. Once activated, PKR phosphorylates eIF2 alpha, leading to inhibition of protein synthesis, growth suppression, and apoptosis induction. The phosphorylation of two sites in the activation loop of the kinase domain, threonines 446 and 451 is critical for high level catalytic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: double stranded RNA activated protein kinase; eIF-2A protein kinase 2; EIF2AK1; EIF2AK2; Eukaryotic translation initiation factor 2-alpha kinase 2; Hypothetical protein PRKR; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; P1/eIF-2A protein kinase; p68 kinase; PKR; PRKR; protein kinase R; Protein kinase RNA-activated; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Tyrosine-protein kinase EIF2AK2
Gene Aliases: EIF2AK1; EIF2AK2; PKR; PPP1R83; PRKR
UniProt ID: (Human) P19525
Entrez Gene ID: (Human) 5610
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.