Immunofluorescent analysis of PLK1 using PLK1 Monoclonal antibody (13E8) (Product# MA1-848) shows staining in WiDr colon carcinoma cells. PLK1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PLK1 (Product# MA1-848) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant protein containing residues 300-400 of human PLK1.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 1 publications below|
MA1-848 detects PLK1 from human, mouse, and rat samples.
MA1-848 has been successfully used in Western blot, immunoprecipitation, and immunofluorescence procedures.
The MA1-848 immunogen is a recombinant protein containing residues 300-400 of human PLK1.
Polo-like Kinase 1 (PLK1) is found to have several functions in mitotic progression. It also has been found to be elevated in a broad range of human tumors. The function of PLK1 appears to be a requirement for proper spindle assembly and other centrosome microtubule events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Small-molecular, non-peptide, non-ATP-competitive polo-like kinase 1 (Plk1) inhibitors with a terphenyl skeleton.
MA1-848 was used in ELISA to synthesize and characterize a series of terphenyl-based small molecule Plk1 inhibitors that are non-ATP competitive
|Mita Y,Noguchi-Yachide T,Ishikawa M,Hashimoto Y||Bioorganic and medicinal chemistry (21:608)||2013|