Immunofluorescence analysis of PLK-1 was done on 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PLK1 (36-298) Mouse Monoclonal Antibody (377100) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full-length human Plk1|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Polo-like kinase 1 induces epithelial-to-mesenchymal transition and promotes epithelial cell motility by activating CRAF/ERK signaling.
37-7100 was used in western blot to research induction of epithelial-to-mesenchymal transition by Polo-like kinase 1 and promotion of epithelial cell motility by activating CRAF/ERK signaling
|Wu J,Ivanov AI,Fisher PB,Fu Z||eLife (5:null)||2016|
Polo-like kinase 1 regulates cell proliferation and is targeted by miR-593* in esophageal cancer.
37-7100 was used in western blot to determine the function and regulation of polo-like kinase 1 in esophageal cancer.
|Ito T,Sato F,Kan T,Cheng Y,David S,Agarwal R,Paun BC,Jin Z,Olaru AV,Hamilton JP,Selaru FM,Yang J,Matsumura N,Shimizu K,Abraham JM,Shimada Y,Mori Y,Meltzer SJ||International journal of cancer (129:2134)||2011|
|Not Applicable||Not Cited||
FOXM1 and polo-like kinase 1 are co-ordinately overexpressed in patients with gastric adenocarcinomas.
37-7100 was used in immunohistochemistry to study co-expression of of FOXM1 and polo-like kinase 1 in gastric adenocarcinoma patients
|Dibb M,Han N,Choudhury J,Hayes S,Valentine H,West C,Sharrocks AD,Ang YS||BMC research notes (8:null)||2015|
||Calmodulin activation of polo-like kinase 1 is required during mitotic entry.||Dai G,Qian Y,Chen J,Meng FL,Pan FY,Shen WG,Zhang SZ,Xue B,Li CJ||Biochemistry and cell biology = Biochimie et biologie cellulaire (91:287)||2013|
|Human||Not Cited||Calmodulin activation of polo-like kinase 1 is required during mitotic entry.||Dai G,Qian Y,Chen J,Meng FL,Pan FY,Shen WG,Zhang SZ,Xue B,Li CJ||Biochemistry and cell biology = Biochimie et biologie cellulaire (91:287)||2013|