|Tested species reactivity||Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal residues K(269) L M G R G T K(276) of PLP.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunocytochemistry (ICC)||See 2 publications below|
PA3-151 detects proteolipid protein in mouse samples.
PA3-151 has been used successfully in Western blot and immunocytochemistry procedures. By Western blot this antibody detects a ~23-26 kDa proteins representing the proteolipid protein and the splice variant DM20 in adult mouse brain samples.
The PA3-151 immunogen is a synthetic peptide corresponding to the C-terminal residues K(269) L M G R G T K(276) of PLP.
Myelin proteins associated with the central nervous system (CNS) are thought to be unique to the CNS, and play a role in the compaction, stabilization, and maintenance of myelin sheaths, as well as in oligodendrocyte development and axonal survival. The proteolipid protein (PLP) and the splice variant DM20 are the predominant myelin proteins in the CNS. PLP is also one of the most extensively studied myelin proteins because of the prevelance of PLP mutations amongst humans and animals. Such mutations can lead to abnormal myelin formation resulting in conditions such as X-linked Pelizaeus-Merzbacher disease and spastic paraplegia type 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Clostridium perfringens Epsilon Toxin Causes Selective Death of Mature Oligodendrocytes and Central Nervous System Demyelination.
PA3-151 was used in immunocytochemistry to elucidate the mechanism by which epsilon-toxin causes white matter damage
|Linden JR,Ma Y,Zhao B,Harris JM,Rumah KR,Schaeren-Wiemers N,Vartanian T||mBio (6:null)||2015|
The myelin proteolipid protein gene modulates apoptosis in neural and non-neural tissues.
PA3-151 was used in immunocytochemistry to study the effect of myelin PLP gene on apoptosis
|Skoff RP,Bessert DA,Cerghet M,Franklin MJ,Rout UK,Nave KA,Carlock L,Ghandour MS,Armant DR||Cell death and differentiation (11:1247)||2004|