Immunolocalization of PMCA4 and CaSRO in bovine corneal epithelium (bCE). (A) Immunolocalization of PMCA4 (green) using MA1-914 on cell membranes of all cells in each layer of bCE. (B) Immunolocalization of CaSRO (red) using MA1-934 on cell membranes of bCE mostly in wing- and squamous cell layers. (C) Merged image.
|Tested species reactivity||Bovine, Human, Pig, Rat|
|Published species reactivity||Rat, Pig, Amphibian, Bovine, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified human erythrocyte PMCA.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||2µg per 10^6 cells|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:500|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-914 specifically detects plasma membrane calcium pump PMCA4 ATPase from human, bovine, porcine and rat tissues.
MA1-914 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects an ~129 kDa and an ~133 kDa protein representing PMCA4b and PMCA4a ATPase, respectively.
The MA1-914 antigen is purified human erythrocyte PMCA ATPase. The epitope for this antibody has been mapped to amino acids 51-75 of PMCA4 ATPase. This sequence is common to both PMCA4a and 4b ATPase.
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump.
There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin (Cat. # MA3-917 and MA3-918).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PMCA4 (ATP2B4) mutation in familial spastic paraplegia causes delay in intracellular calcium extrusion.
MA1-914 was used in western blot to describe the functional effect of a novel missense mutation in familial spastic paraplegia
|Ho PW,Pang SY,Li M,Tse ZH,Kung MH,Sham PC,Ho SL||Brain and behavior (5:null)||2015|
Vesicular transfer of membrane components to bovine epididymal spermatozoa.
MA1-914 was used in immunohistochemistry and western blot to study the ability of epididymosomes to transfer lipidic and proteinaceous membrane components to bovine sperm cells
|Schwarz A,Wennemuth G,Post H,Brandenburger T,Aumüller G,Wilhelm B||Cell and tissue research (353:549)||2013|
Distinct regulation of cytoplasmic calcium signals and cell death pathways by different plasma membrane calcium ATPase isoforms in MDA-MB-231 breast cancer cells.
MA1-914 was used in western blot to study the roles of different plasma membrane calcium ATPase isoforms in cytoplasmic Ca2+ signaling and cell death
|Curry MC,Luk NA,Kenny PA,Roberts-Thomson SJ,Monteith GR||The Journal of biological chemistry (287:28598)||2012|
The plasma membrane Ca2+-ATPase isoform 4 is localized in lipid rafts of cerebellum synaptic plasma membranes.
MA1-914 was used in western blot to localize the plasma membrane calcium-ATPase isoform 4.
|Sepúlveda MR,Berrocal-Carrillo M,Gasset M,Mata AM||The Journal of biological chemistry (281:447)||2006|
Regional distribution of Na,K-ATPase activity in porcine lens epithelium.
MA1-914 was used in western blot to investigate the sodium/potassium ATPase protein expression in porcine lens epithelium.
|Tamiya S,Dean WL,Paterson CA,Delamere NA||Investigative ophthalmology and visual science (44:4395)||2003|
Use of expression mutants and monoclonal antibodies to map the erythrocyte Ca2+ pump.
MA1-914 was used in western blot to investigate the human erythrocyte caclium pump through the use of mutants and monoclonal antibodies
|Adamo HP,Caride AJ,Penniston JT||The Journal of biological chemistry (267:14244)||1992|
Compartmentalized expression of three novel sarco/endoplasmic reticulum Ca2+ATPase 3 isoforms including the switch to ER stress, SERCA3f, in non-failing and failing human heart.
MA1-914 was used in immunohistochemistry to study the expression of three novel Ca2+-ATPase 3 isoforms in failing and non-failing human heart
|Dally S,Monceau V,Corvazier E,Bredoux R,Raies A,Bobe R,del Monte F,Enouf J||Cell calcium (45:144)||2009|
Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum).
MA1-914 was used in immunohistochemistry to investigate how cytoplasmic free calcium in neurons from the salamander retina is regulated
|Krizaj D,Liu X,Copenhagen DR||The Journal of comparative neurology (475:463)||2004|
Caytaxin deficiency disrupts signaling pathways in cerebellar cortex.
MA1-914 was used in immunocytochemistry to investigate the role of caytaxin for signaling pathways in cerebellar cortex
|Xiao J,Gong S,Ledoux MS||Neuroscience (144:439)||2007|