MA1-914 specifically detects plasma membrane calcium pump PMCA4 ATPase from human, bovine, porcine and rat tissues.
MA1-914 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects an ~129 kDa and an ~133 kDa protein representing PMCA4b and PMCA4a ATPase, respectively.
The MA1-914 antigen is purified human erythrocyte PMCA ATPase. The epitope for this antibody has been mapped to amino acids 51-75 of PMCA4 ATPase. This sequence is common to both PMCA4a and 4b ATPase.
The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump.
There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin (Cat. # MA3-917 and MA3-918).
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Protein Aliases: ATPase, Ca++ transporting, plasma membrane 4; ATPase, Ca++ transporting, plasma membrane 4 ATP synthase, H+ transporting, mitochondrial F1; Matrix-remodeling-associated protein 1; plasma membrane Ca2+-ATPases 4; plasma membrane calcium ATPase; Plasma membrane calcium ATPase isoform 4; plasma membrane calcium pump; Plasma Membrane Calcium Pump ATPase 4; Plasma membrane calcium pump isoform 4; Plasma membrane calcium-transporting ATPase 4; PMCA4; sarcolemmal calcium pump
Gene Aliases: ATP2B2; ATP2B4; BOS_15586; MXRA1; PMCA4; PMCA4b; PMCA4x