Invitrogen

PMCA4 ATPase Monoclonal Antibody (JA9)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

9 Published Figures

17 References

View all (5) PMCA4 ATPase antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

PMCA4 ATPase Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 14

PMCA4 ATPase Antibody (MA1-914) in ICC/IF

Immunolocalization of PMCA4 and CaSRO in bovine corneal epithelium (bCE). (A) Immunolocalization of PMCA4 (green) using Product # MA1-914 on cell membranes of all cells in each layer of bCE. (B) Immunolocalization of CaSRO (red) using Product # MA1-934 on cell membranes of bCE mostly in wing- and squamous cell layers. (C) Merged image.

Product Image Gallery

PMCA4 ATPase Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing PMCA4 ATPase (Product # MA1-914) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

PMCA4 ATPase Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using PMCA4 ATPase monoclonal antibody (Product # MA1-914) in Immunocytochemistry and Immunohistochemistry

PMCA4 ATPase Antibody in Immunohistochemistry (IHC) — Published figure using PMCA4 ATPase monoclonal antibody (Product # MA1-914) in Immunohistochemistry

PMCA4 ATPase Antibody in Western Blot (WB) — Figure 6 Adaptations of calcium (Ca 2+ ) handling in tamoxifen-inducible sodium-Ca 2+ exchanger isoform 1 (NCX1) knockout (KO) mice. A , Representative traces (left) and summary data (right) of Ca 2+ transients recorded using Fura-2 acetoxymethyl ester in control mice (Ctl, black) and tamoxifen-inducible NCX1 KO mice at 1 week (Tm_1w, red) and 4 weeks (Tm_4w, blue) post-tamoxifen. B , Representative confocal line scans (left) and summary data (right) of Ca 2+ wave frequency, velocity (mum/s), and amplitude (DeltaF/F 0 ) recorded in isolated ventricular myocytes loaded with Fluo-4 acetoxymethyl ester from control mice (Ctl, N=66 cells from 8 mice) and mice 1 week (Tm_1w, N=47 cells from 4 mice) and 4 weeks (Tm_4w, N=62 cells from 6 mice) after tamoxifen injection. C , Representative Western blots (left) and summary data (right) from ventricular cardiomyocytes showing sarcoplasmic reticulum Ca 2+ ATPase 2 (SERCA2), phospholamban (PLB), plasma membrane calcium ATPase 4 (PMCA4), Ca v 1.2 (calcium channel voltage-dependent L type alpha 1C subunit), calcium/calmodulin-dependent protein kinase II (CaMKII), and phosphorylated CaMKII (P-CaMKII) expression. Mean data normalized to GAPDH. Data are expressed as mean+-SEM (1-way ANOVA) or median and interquartile range (Kruskal-Wallis). Data from control mice (Ctl, N=9) and mice 1 week (Tm_1w, N=6) and 4 weeks (Tm_4w, N=6 mice) after tamoxifen injection. * P <0.05, ** P <0.01, *** P <0.001 by 1-way ANOVA (p-CaMKII/CaMKII ratio, SERCA2/PLB

PMCA4 ATPase Antibody — Figure 3 siRNA knockdown of PMCA4 in MIA PaCa-2 PDAC cells. ( A ) Representative Western immunoblotting comparing PMCA4 protein expression in MIA PaCa-2 cells after 24-96 h of treatment with siNT (control) and siPMCA4 (PMCA4 knockdown). ( B ) PMCA4 protein expression of siPMCA4 treated cells was quantified from Western blot bands and normalized to beta-Actin (n = 3). Statistical comparison was made using the Kruskal-Wallis test with Dunn's multiple comparison test, comparing 0-hour control to 24-96 h post-siPMCA4 treatment conditions. ( C ) The relative expression of ATP2B4 (PMCA4 mRNA) and ( D ) ATP2B4b (PMCA4b mRNA) were examined after 48 h of siRNA treatment by RT-qPCR. The expressions of target mRNA were normalized to S18 rRNA and expressed as 2 -(DeltaDeltaCtau) . Data are shown as mean +- SEM. (n = 4, 4 replicates per treatment condition). Comparisons were made between siNT control and siPMCA4 treated cells at matching time points post-drug treatment using Mann-Whitney U-test (Unpaired, non-parametric rank test). * and ** represents statistically significant difference where p < 0.05 and p < 0.01, respectively. {KD}

Product Details

MA1-914

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 11 publications 11 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:500

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:500

Immunocytochemistry (ICC/IF)

Assay-dependent

View 3 publications 3 publications

Flow Cytometry (Flow)

2 µg / 10^6 cells

ELISA (ELISA)

1:15,000

Product Specifications

Species Reactivity

Bovine, Human, Pig, Rat

Published species

Amphibian, Bovine, Human, Mouse, Pig, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

JA9

Immunogen

Purified human erythrocyte PMCA.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2061596

Product Specific Information

MA1-914 specifically detects plasma membrane calcium pump PMCA4 ATPase from human, bovine, porcine and rat tissues.

MA1-914 has been successfully used in Western blot, immunohistochemistry and ELISA procedures. By Western blot, this antibody detects an ~129 kDa and an ~133 kDa protein representing PMCA4b and PMCA4a ATPase, respectively.

The MA1-914 antigen is purified human erythrocyte PMCA ATPase. The epitope for this antibody has been mapped to amino acids 51-75 of PMCA4 ATPase. This sequence is common to both PMCA4a and 4b ATPase.

Target Information

The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/K+ pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin (Cat. # MA3-917 and MA3-918).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Acute Genetic Ablation of Cardiac Sodium/Calcium Exchange in Adult Mice: Implications for Cardiomyocyte Calcium Regulation, Cardioprotection, and Arrhythmia.

Journal of the American Heart Association

Lotteau S,Zhang R,Hazan A,Grabar C,Gonzalez D,Aynaszyan S,Philipson KD,Ottolia M,Goldhaber JI

Published figure using PMCA4 ATPase monoclonal antibody (Product # MA1-914) in Western Blot

Tue Sep 07 00:00:00 EDT 2021

Acute Genetic Ablation of Cardiac Sodium/Calcium Exchange in Adult Mice: Implications for Cardiomyocyte Calcium Regulation, Cardioprotection, and Arrhythmia.

Journal of the American Heart Association

Lotteau S,Zhang R,Hazan A,Grabar C,Gonzalez D,Aynaszyan S,Philipson KD,Ottolia M,Goldhaber JI

Published figure using PMCA4 ATPase monoclonal antibody (Product # MA1-914) in Western Blot

Tue Sep 07 00:00:00 EDT 2021

Molecular and Electrophysiological Analyses of ATP2B4 Gene Variants in Bilateral Adrenal Hyperaldosteronism.

Hormones & cancer

Hattangady NG,Foster J,Lerario AM,Ponce-Balbuena D,Rege J,Monticone S,Rainey WE,Mulatero P,Else T

MA1-914 was used in Immunocytochemistry to identify rare gene variants in ATP2B4, in a cohort of patients with bilateral hyperaldosteronism.

Sat Feb 01 00:00:00 EST 2020

Metabolic regulation of calcium pumps in pancreatic cancer: role of phosphofructokinase-fructose-bisphosphatase-3 (PFKFB3).

Cancer & metabolism

Richardson DA,Sritangos P,James AD,Sultan A,Bruce JIE

Published figure using PMCA4 ATPase monoclonal antibody (Product # MA1-914) in Western Blot

Wed Apr 13 00:00:00 EDT 2022

Plasma Membrane Ca²⁺ ATPase Isoform 4 (PMCA4) Has an Important Role in Numerous Hallmarks of Pancreatic Cancer.

Cancers

Sritangos P,Pena Alarcon E,James AD,Sultan A,Richardson DA,Bruce JIE

Published figure using PMCA4 ATPase monoclonal antibody (Product # MA1-914) in Western Blot

Thu Jan 16 00:00:00 EST 2020

Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.

Frontiers in cellular neuroscience

Lisek M,Ferenc B,Studzian M,Pulaski L,Guo F,Zylinska L,Boczek T

MA1-914 was used in Western Blotting to characterize brain region-specific effects of ketamine on PMCA activity.

Thu Oct 01 00:00:00 EDT 2020

PMCA4 (ATP2B4) mutation in familial spastic paraplegia causes delay in intracellular calcium extrusion.

Brain and behavior

Ho PW,Pang SY,Li M,Tse ZH,Kung MH,Sham PC,Ho SL

Published figure using PMCA4 ATPase monoclonal antibody (Product # MA1-914) in Western Blot

Wed Apr 01 00:00:00 EDT 2015

Vesicular transfer of membrane components to bovine epididymal spermatozoa.

Cell and tissue research

Schwarz A,Wennemuth G,Post H,Brandenburger T,Aumüller G,Wilhelm B

MA1-914 was used in immunohistochemistry and western blot to study the ability of epididymosomes to transfer lipidic and proteinaceous membrane components to bovine sperm cells

Sun Sep 01 00:00:00 EDT 2013

Distinct regulation of cytoplasmic calcium signals and cell death pathways by different plasma membrane calcium ATPase isoforms in MDA-MB-231 breast cancer cells.

The Journal of biological chemistry

Curry MC,Luk NA,Kenny PA,Roberts-Thomson SJ,Monteith GR

MA1-914 was used in western blot to study the roles of different plasma membrane calcium ATPase isoforms in cytoplasmic Ca2+ signaling and cell death

Fri Aug 17 00:00:00 EDT 2012

Compartmentalized expression of three novel sarco/endoplasmic reticulum Ca2+ATPase 3 isoforms including the switch to ER stress, SERCA3f, in non-failing and failing human heart.

Cell calcium

Dally S,Monceau V,Corvazier E,Bredoux R,Raies A,Bobe R,del Monte F,Enouf J

MA1-914 was used in immunohistochemistry to study the expression of three novel Ca2+-ATPase 3 isoforms in failing and non-failing human heart

Sun Feb 01 00:00:00 EST 2009

Muscarinic-induced recruitment of plasma membrane Ca2+-ATPase involves PSD-95/Dlg/Zo-1-mediated interactions.

The Journal of biological chemistry

Kruger WA,Yun CC,Monteith GR,Poronnik P

MA1-914 was used in Western Blotting to explore the molecular mechanisms that underpin the trafficking of plasma membrane Ca2+-ATPases to the membrane during Ca2+ signalling.

Fri Jan 16 00:00:00 EST 2009

Caytaxin deficiency disrupts signaling pathways in cerebellar cortex.

Neuroscience

Xiao J,Gong S,Ledoux MS

Published figure using PMCA4 ATPase monoclonal antibody (Product # MA1-914) in Immunofluorescence

Fri Jan 19 00:00:00 EST 2007

Caytaxin deficiency disrupts signaling pathways in cerebellar cortex.

Neuroscience

Xiao J,Gong S,Ledoux MS

Published figure using PMCA4 ATPase monoclonal antibody (Product # MA1-914) in Immunofluorescence

Fri Jan 19 00:00:00 EST 2007

Plasma membrane calcium pumps in mouse olfactory sensory neurons.

Chemical senses

Weeraratne SD,Valentine M,Cusick M,Delay R,Van Houten JL

Published figure using PMCA4 ATPase monoclonal antibody (Product # MA1-914) in Immunofluorescence

Sun Oct 01 00:00:00 EDT 2006

The plasma membrane Ca2+-ATPase isoform 4 is localized in lipid rafts of cerebellum synaptic plasma membranes.

The Journal of biological chemistry

Sepúlveda MR,Berrocal-Carrillo M,Gasset M,Mata AM

MA1-914 was used in western blot to localize the plasma membrane calcium-ATPase isoform 4.

Fri Jan 06 00:00:00 EST 2006

Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum).

The Journal of comparative neurology

Krizaj D,Liu X,Copenhagen DR

MA1-914 was used in immunohistochemistry to investigate how cytoplasmic free calcium in neurons from the salamander retina is regulated

Mon Aug 02 00:00:00 EDT 2004

Regional distribution of Na,K-ATPase activity in porcine lens epithelium.

Investigative ophthalmology & visual science

Tamiya S,Dean WL,Paterson CA,Delamere NA

MA1-914 was used in western blot to investigate the sodium/potassium ATPase protein expression in porcine lens epithelium.

Wed Oct 01 00:00:00 EDT 2003

Use of expression mutants and monoclonal antibodies to map the erythrocyte Ca2+ pump.

The Journal of biological chemistry

Adamo HP,Caride AJ,Penniston JT

MA1-914 was used in western blot to investigate the human erythrocyte caclium pump through the use of mutants and monoclonal antibodies

Wed Jul 15 00:00:00 EDT 1992

Bioinformatics

Protein Aliases: ATPase, Ca++ transporting, plasma membrane 4; ATPase, Ca++ transporting, plasma membrane 4 ATP synthase, H+ transporting, mitochondrial F1; ATPase, Ca++ transporting, plasma membrane 4 tv2; Matrix-remodeling-associated protein 1; plasma membrane Ca2+-ATPases 4; plasma membrane calcium ATPase; plasma membrane calcium ATPase 4; Plasma membrane calcium ATPase isoform 4; plasma membrane calcium pump; Plasma membrane calcium pump isoform 4; plasma membrane calcium-pumping ATP-ase; Plasma membrane calcium-transporting ATPase 4; PMCA4; PMCA4b; sarcolemmal calcium pump

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Gene Aliases: ATP2B2; ATP2B4; MXRA1; PMCA4; PMCA4b; PMCA4x

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UniProt ID: (Bovine) D3K0R6, (Human) P23634, (Rat) Q64542

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Entrez Gene ID: (Bovine) 282146, (Human) 493, (Pig) 100623420, (Rat) 29600

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Molecular Function: primary active transporter

Function(s)

Process(es)

calmodulin binding ATP binding protein phosphatase 2B binding nitric-oxide synthase inhibitor activity metal ion binding calcium-dependent protein binding nitric-oxide synthase binding calcium-transporting ATPase activity protein binding sodium channel regulator activity PDZ domain binding scaffold protein binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PMCA4 ATPase Monoclonal Antibody (JA9)

PMCA4 ATPase Antibody (MA1-914) in ICC/IF

Product Details

MA1-914

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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