Invitrogen

PMEL Monoclonal Antibody (HMB45)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

37 References

View all (39) PMEL antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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PMEL Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PMEL was performed using 70% confluent log phase SK-MEL-5 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PMEL Monoclonal Antibody (HMB45) (Product # MA1-34759) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic(vesicles) localization. Panel e represents A-375 cells having no expression of PMEL. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PMEL Antibody in Western Blot (WB) — Western blot was performed using Anti-PMEL Monoclonal Antibody (HMB45) (Product # MA1-34759) and a 32-35kDa band corresponding to PMEL was observed across in SK-MEL-5 in comparison to A-375 and SK-BR-3. Whole cell extracts (30 µg lysate) of SK-MEL-5 (Lane 1), A-375 (Lane 2) and SK-BR-3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

PMEL Antibody — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of PMEL was observed in SK-MEL-5 in comparison to A-375 and SK-BR-3 using Anti-PMEL Monoclonal Antibody (HMB45) (Product # MA1-34759) in Western Blot. {RE}

Product Details

MA1-34759

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 16 publications 16 publications
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	5 µg/mL	View 12 publications 12 publications
Neutralization (Neu)	-	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Bovine, Human, Mouse
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	HMB45
Immunogen	The extract of pigmented melanoma metastases from lymph nodes.
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 0.2% BSA
Contains	0.09% sodium azide
Storage conditions	4° C, do not freeze
RRID	AB_1955861

Product Specific Information

This antibody reacts with an antigen present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells.This antibody reacts with melanomas and tumors showing melanoma/melanocytic differentiation. It reacts with junctional and blue nevus cells. Non-melanocytic cells do not react with this antibody.

Target Information

Melanoma is a malignant tumor of melanocytes which are found predominantly in skin but also in the bowel and the eye (see uveal melanoma). It is one of the rarer types of skin cancer but causes the majority of skin cancer related deaths.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Two novel TSC2 mutations in renal epithelioid angiomyolipoma sensitive to everolimus.

Cancer biology & therapy

Wang T,Xie S,Luo R,Shi L,Bai P,Wang X,Wan R,Deng J,Wu Z,Li W,Xiao W,Wang Y,Chen B,Zhang K,Xing J

MA1-34759 was used in Immunohistochemistry to identify novel TSC2 mutations in one Chinese renal epithelioid AML patient: c.2652C>A; c.2688G>A based on sequencing result from biopsy tissue and report the outcomes of everolimus treatment.

Mon Mar 29 00:00:00 EDT 2021

A challenging case of ocular melanoma.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

Costache M,Dumitru AV,Pătraşcu OM,Popa-Cherecheanu DA,Bădilă P,Miu JC,Procop A,Popa M,Tampa MŞ,Sajin M,Simionescu O,Cîrstoiu MM

MA1-34759 was used in immunohistochemistry to present a case of highly aggressive ocular melanoma

Tue May 10 00:00:00 EDT 2016

High fat diet increases melanoma cell growth in the bone marrow by inducing osteopontin and interleukin 6.

Oncotarget

Chen GL,Luo Y,Eriksson D,Meng X,Qian C,Bäuerle T,Chen XX,Schett G,Bozec A

MA1-34759 was used in immunohistochemistry - paraffin section to study an increasing melanoma cell growth in the bone marrow by a high fat diet that induces osteopontin and interleukin 6

Tue May 03 00:00:00 EDT 2016

Targeting histone deacetylase 6 mediates a dual anti-melanoma effect: Enhanced antitumor immunity and impaired cell proliferation.

Molecular oncology

Woan KV,Lienlaf M,Perez-Villaroel P,Lee C,Cheng F,Knox T,Woods DM,Barrios K,Powers J,Sahakian E,Wang HW,Canales J,Marante D,Smalley KSM,Bergman J,Seto E,Kozikowski A,Pinilla-Ibarz J,Sarnaik A,Celis E,Weber J,Sotomayor EM,Villagra A

MA1-34759 was used in western blot to determine which HDAC to target to specifically inhibit metastatic melanoma

Sat Aug 01 00:00:00 EDT 2015

ASPL-TFE3 translocation in vulvovaginal alveolar soft part sarcoma.

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists

Jabbour MN,Seoud M,Al-Ahmadie H,Abdul-Karim FW,Zaatari GS

MA1-34759 was used in immunohistochemistry to report on a case of vulvovaginal alveolar soft part sarcoma

Thu May 01 00:00:00 EDT 2014

Cardiac Epithelioid PEComa: Report of Two Cases and Review of the Literature.

Case reports in medicine

Niu H,Wang FW,Zhang PJ,Bing Z

MA1-34759 was used in immunohistochemistry - paraffin section to present two cases of epithelioid PEComas

Fri Sep 14 00:00:00 EDT 2012

Targeting BRAFV600E in an inducible murine model of melanoma.

The American journal of pathology

Hooijkaas AI,Gadiot J,van der Valk M,Mooi WJ,Blank CU

MA1-34759 was used in immunohistochemistry to study the effects of targeting BRAF(V600E) on melanoma growth in an inducible murine model

Sat Sep 01 00:00:00 EDT 2012

Differential recognition of a dileucine-based sorting signal by AP-1 and AP-3 reveals a requirement for both BLOC-1 and AP-3 in delivery of OCA2 to melanosomes.

Molecular biology of the cell

Sitaram A,Dennis MK,Chaudhuri R,De Jesus-Rojas W,Tenza D,Setty SR,Wood CS,Sviderskaya EV,Bennett DC,Raposo G,Bonifacino JS,Marks MS

MA1-34759 was used in immunocytochemistry to study the delivery of OCA2 to melanosomes and the distinct roles played by AP-1 and AP-3

Wed Aug 01 00:00:00 EDT 2012

Genetic ablation of SOX18 function suppresses tumor lymphangiogenesis and metastasis of melanoma in mice.

Cancer research

Duong T,Proulx ST,Luciani P,Leroux JC,Detmar M,Koopman P,Francois M

MA1-34759 was used in immunohistochemistry to study the role of SOX18 function function in tumor lymphangiogenesis and metastasis of melanoma in mice

Fri Jun 15 00:00:00 EDT 2012

Sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression: report of a case and literature review.

Diagnostic pathology

Yin WH,Li J,Chan JK

MA1-34759 was used in immunohistochemistry to report on a case of sporadic haemangioblastoma of the kidney with rhabdoid features and focal CD10 expression

Thu Apr 12 00:00:00 EDT 2012

Effect of PUVA therapy on melanocytes and keratinocytes in non-segmental vitiligo: histopathological, immuno-histochemical and ultrastructural study.

Photodermatology, photoimmunology & photomedicine

Anbar TS,El-Sawy AE,Attia SK,Barakat MT,Moftah NH,El-Ammawy TS,Abdel-Rahman AT,El-Tonsy MH

MA1-34759 was used in immunohistochemistry to study the effects of PUVA treatment on melanocytes and keratinocytes from patients with non-segmental vitiligo

Wed Feb 01 00:00:00 EST 2012

Cellular and clinical report of new Griscelli syndrome type III cases.

Pigment cell & melanoma research

Westbroek W,Klar A,Cullinane AR,Ziegler SG,Hurvitz H,Ganem A,Wilson K,Dorward H,Huizing M,Tamimi H,Vainshtein I,Berkun Y,Lavie M,Gahl WA,Anikster Y

MA1-34759 was used in immunocytochemistry to report on new cases of Griscelli syndrome type III

Sun Jan 01 00:00:00 EST 2012

Mutations in or near the transmembrane domain alter PMEL amyloid formation from functional to pathogenic.

PLoS genetics

Watt B,Tenza D,Lemmon MA,Kerje S,Raposo G,Andersson L,Marks MS

MA1-34759 was used in western blot to investigate the role of PMEL mutations in physiological and pathological amyloid fibril formation

Thu Sep 01 00:00:00 EDT 2011

Proprotein convertases process Pmel17 during secretion.

The Journal of biological chemistry

Leonhardt RM,Vigneron N,Rahner C,Cresswell P

MA1-34759 was used in immunocytochemistry and western blot to study Pmel17 processing in melanocytes

Fri Mar 18 00:00:00 EDT 2011

Endoscopic ultrasound-guided fine-needle aspiration of intrathoracic and intra-abdominal spindle cell and mesenchymal lesions.

Cancer cytopathology

Bean SM,Baker A,Eloubeidi M,Eltoum I,Jhala N,Crowe R,Jhala D,Chhieng DC

MA1-34759 was used in immunocytochemistry to confirm the diagnostic value of endoscopic ultrasound-guided fine-needle aspiration

Fri Feb 25 00:00:00 EST 2011

Primary myoepithelial carcinoma of the larynx: case report and review of the literature.

Pathology, research and practice

Yu G,Qu G,Kong L,Pan X,Wang W,Lv J

MA1-34759 was used in immunohistochemistry to report on a case of primary myoepithelial carcinoma of the larynx

Tue Feb 15 00:00:00 EST 2011

Microscopic uterine lymphangioleiomyomatosis perivascular epithelioid cell neoplasm: a case report with the earliest manifestation of this enigmatic neoplasm.

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists

Clay MR,Gibson P,Lowell J,Cooper K

MA1-34759 was used in immunohistochemistry to report a clinical case of microscopic lymphangioleiomyomatosis perivascular epithelioid cell neoplasm in uterus

Sat Jan 01 00:00:00 EST 2011

Clinical and cellular characterisation of Hermansky-Pudlak syndrome type 6.

Journal of medical genetics

Huizing M,Pederson B,Hess RA,Griffin A,Helip-Wooley A,Westbroek W,Dorward H,O'Brien KJ,Golas G,Tsilou E,White JG,Gahl WA

MA1-34759 was used in immunocytochemistry to study the clinical and cellular characteristics of Hermansky-Pudlak syndrome subtype 6

Tue Dec 01 00:00:00 EST 2009

Cutaneous metastases of malignant melanoma--how difficult can it be?

Romanian journal of internal medicine = Revue roumaine de medecine interne

Zurac S,Andrei R,Petsakos G,Nichita L,Bastian A,Micu G,Gramadă E,Popp C,Stăniceanu F,Petrescu S,Negroiu G,Giurcăneanu D,Chiţu V

MA1-34759 was used in immunohistochemistry to investigate the differentiating features between primary melanoma and its cutaneous metastasis

Fri Jun 26 00:00:00 EDT 2009

Patterns of repigmentation in two cases of hypopigmented type of vitiligo.

Photodermatology, photoimmunology & photomedicine

Anbar TS,El-Sawy AE,Attia SK,Moftah NH,El-Tonsy MH

MA1-34759 was used in immunohistochemistry to study patterns of repigmentation in two cases of hypopigmented type of vitiligo

Mon Jun 01 00:00:00 EDT 2009

Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.

The American journal of surgical pathology

Mukhopadhyay S,El-Zammar OA,Katzenstein AL

MA1-34759 was used in immunohistochemistry to study pulmonary meningothelial-like nodules

Wed Apr 01 00:00:00 EDT 2009

Cell-specific ATP7A transport sustains copper-dependent tyrosinase activity in melanosomes.

Nature

Setty SR,Tenza D,Sviderskaya EV,Bennett DC,Raposo G,Marks MS

MA1-34759 was used in western blot to investigate the role of cell-specific ATP7A transport in copper-dependent tyrosinase activity of melanosomes

Thu Aug 28 00:00:00 EDT 2008

Vacuolar-type H(+)-ATPase with the a3 isoform is the proton pump on premature melanosomes.

Cell and tissue research

Tabata H,Kawamura N,Sun-Wada GH,Wada Y

MA1-34759 was used in immunohistochemistry to identify the proton pump on immature melanosomes as the vacuolar H(+)-ATPase a3 isoform

Sun Jun 01 00:00:00 EDT 2008

Glycolipid-dependent sorting of melanosomal from lysosomal membrane proteins by lumenal determinants.

Traffic (Copenhagen, Denmark)

Groux-Degroote S,van Dijk SM,Wolthoorn J,Neumann S,Theos AC,De Mazière AM,Klumperman J,van Meer G,Sprong H

MA1-34759 was used in immunocytochemistry and western blot to study glycolipid-dependent sorting of melanosomal from lysosomal membrane proteins

Sun Jun 01 00:00:00 EDT 2008

A novel missense mutation (G43S) in the switch I region of Rab27A causing Griscelli syndrome.

Molecular genetics and metabolism

Westbroek W,Tuchman M,Tinloy B,De Wever O,Vilboux T,Hertz JM,Hasle H,Heilmann C,Helip-Wooley A,Kleta R,Gahl WA

MA1-34759 was used in immunocytochemistry to investigate the role of Rab27A specific mutation in Griscelli syndrome

Sun Jun 01 00:00:00 EDT 2008

Melanocytes expressing MC1R polymorphisms associated with red hair color have altered MSH-ligand activated pigmentary responses in coculture with keratinocytes.

Journal of cellular physiology

Roberts DW,Newton RA,Leonard JH,Sturm RA

MA1-34759 was used in western blot to study the behavior and functional abilities of melanocytes expressing MC1R red hair color variants

Thu May 01 00:00:00 EDT 2008

Rab7 regulates maturation of melanosomal matrix protein gp100/Pmel17/Silv.

The Journal of investigative dermatology

Kawakami A,Sakane F,Imai S,Yasuda S,Kai M,Kanoh H,Jin HY,Hirosaki K,Yamashita T,Fisher DE,Jimbow K

MA1-34759 was used in western blot to study the regulation of the melanosomal matrix protein gp100 by Rab7

Tue Jan 01 00:00:00 EST 2008

Melanosis in a Holstein heifer.

Journal of veterinary medicine. A, Physiology, pathology, clinical medicine

Oruç E

MA1-34759 was used in immunohistochemistry to investigate histological features in a Holstein heifer with melanosis

Thu Nov 01 00:00:00 EDT 2007

Cellular defects in Chediak-Higashi syndrome correlate with the molecular genotype and clinical phenotype.

The Journal of investigative dermatology

Westbroek W,Adams D,Huizing M,Koshoffer A,Dorward H,Tinloy B,Parkes J,Helip-Wooley A,Kleta R,Tsilou E,Duvernay P,Digre KB,Creel DJ,White JG,Boissy RE,Gahl WA

MA1-34759 was used in immunohistochemistry to study the correlation between cellular defects, genotype and clinical phenotype in Chediak-Higashi syndrome

Thu Nov 01 00:00:00 EDT 2007

Improper trafficking of melanocyte-specific proteins in Hermansky-Pudlak syndrome type-5.

The Journal of investigative dermatology

Helip-Wooley A,Westbroek W,Dorward HM,Koshoffer A,Huizing M,Boissy RE,Gahl WA

MA1-34759 was used in immunocytochemistry to study the trafficking of melanocyte-specific proteins in Hermansky-Pudlak syndrome type-5 melanocytes

Fri Jun 01 00:00:00 EDT 2007

Characterization of t(6;11)(p21;q12) in a renal-cell carcinoma of an adult patient.

Genes, chromosomes & cancer

Pecciarini L,Cangi MG,Lo Cunsolo C,Macri' E,Dal Cin E,Martignoni G,Doglioni C

MA1-34759 was used in immunohistochemistry to report a clinical case of t(6;11)(p21;q12) renal-cell carcinoma

Tue May 01 00:00:00 EDT 2007

BLOC-1 is required for cargo-specific sorting from vacuolar early endosomes toward lysosome-related organelles.

Molecular biology of the cell

Setty SR,Tenza D,Truschel ST,Chou E,Sviderskaya EV,Theos AC,Lamoreux ML,Di Pietro SM,Starcevic M,Bennett DC,Dell'Angelica EC,Raposo G,Marks MS

MA1-34759 was used in immunocytochemistry to investigate the role of BLOC-1 in melanosome maturation

Thu Mar 01 00:00:00 EST 2007

Dual loss of ER export and endocytic signals with altered melanosome morphology in the silver mutation of Pmel17.

Molecular biology of the cell

Theos AC,Berson JF,Theos SC,Herman KE,Harper DC,Tenza D,Sviderskaya EV,Lamoreux ML,Bennett DC,Raposo G,Marks MS

MA1-34759 was used in immunocytochemistry to examine the mechanism for the coat hypopigmentation

Tue Aug 01 00:00:00 EDT 2006

Accumulation of low-avidity anti-melanocortin receptor 1 (anti-MC1R) CD8+ T cells in the lesional skin of a patient with melanoma-related depigmentation.

Melanoma research

Wankowicz-Kalinska A,Mailliard RB,Olson K,Graham F,Edington H,Kirkwood JM,Martinek S,Das PK,Storkus WJ

MA1-34759 was used in immunohistochemistry to investigat specific CD8+ tissue-infiltrating T cells in a patient with melanoma

Sat Apr 01 00:00:00 EST 2006

Mannose receptor targeting of tumor antigen pmel17 to human dendritic cells directs anti-melanoma T cell responses via multiple HLA molecules.

Journal of immunology (Baltimore, Md. : 1950)

Ramakrishna V,Treml JF,Vitale L,Connolly JE,O'Neill T,Smith PA,Jones CL,He LZ,Goldstein J,Wallace PK,Keler T,Endres MJ

MA1-34759 was used in blocking or activating experiment to study the T cell immune response against melanoma elicited through conjugation of melanoma antigen with mannose receptor antibody

Mon Mar 01 00:00:00 EST 2004

A novel splice variant of Pmel17 expressed by human melanocytes and melanoma cells lacking some of the internal repeats.

The Journal of investigative dermatology

Nichols SE,Harper DC,Berson JF,Marks MS

MA1-34759 was used in immunocytochemistry to study the alternative splicing of Pmel17 in human melanocytes and melanoma cells

Wed Oct 01 00:00:00 EDT 2003

Proprotein convertase cleavage liberates a fibrillogenic fragment of a resident glycoprotein to initiate melanosome biogenesis.

The Journal of cell biology

Berson JF,Theos AC,Harper DC,Tenza D,Raposo G,Marks MS

MA1-34759 was used in immunocytochemistry to investigate the role of proprotein convertase cleavage in melanosome biogenesis

Mon May 12 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: 95 kDa melanocyte-specific secreted glycoprotein; 95kDa melanocyte-specific secreted glycoprotein; M-alpha; M-beta; ME20-M; ME20-S; ME20M; Melanocyte lineage specific antigen GP100; melanocyte protein mel 17; Melanocyte protein PMEL; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; melanosomal matrix protein17; P1; P100; P26; Pmel 17; Premelanosome protein; Secreted melanoma-associated ME20 antigen; Silver locus protein homolog; silver, mouse, homolog of

Gene Aliases: D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL; PMEL17; SI; SIL; SILV

UniProt ID: (Human) P40967

Entrez Gene ID: (Human) 6490

Molecular Function: membrane-bound signaling molecule