Invitrogen

PMEL Monoclonal Antibody (HMB45)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

36 References

View all (29) PMEL antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

PMEL Antibody in Immunocytochemistry (ICC) — Immunofluorescence analysis of PMEL was performed using 70% confluent log phase SK-MEL-5 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PMEL Monoclonal Antibody (HMB45) (Product # MA1-34759) at 5 ug/ml in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic(vesicles) localization. Panel e represents A-375 cells having no expression of PMEL. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PMEL Antibody in Western Blot (WB) — Western blot was performed using Anti-PMEL Monoclonal Antibody (HMB45) (Product # MA1-34759) and a 32-35kDa band corresponding to PMEL was observed across in SK-MEL-5 in comparison to A-375 and SK-BR-3. Whole cell extracts (30 µg lysate) of SK-MEL-5 (Lane 1), A-375 (Lane 2) and SK-BR-3 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

PMEL Antibody in Relative expression — Antibody specificity was demonstrated by the detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of PMEL was observed in SK-MEL-5 in comparison to A-375 and SK-BR-3 using Anti-PMEL Monoclonal Antibody (HMB45) (Product # MA1-34759) in Western Blot. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

5 µg/mL

View 12 publications 12 publications

Immunofluorescence (IF)

5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 2 publications 2 publications

Western Blot (WB)

1:1,000

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 15 publications 15 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species

Human

Published species

Bovine, Human, Mouse

Expression System

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

HMB45

Immunogen

The extract of pigmented melanoma metastases from lymph nodes.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

RRID

AB_1955861

Product Specific Information

This antibody reacts with an antigen present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells.This antibody reacts with melanomas and tumors showing melanoma/melanocytic differentiation. It reacts with junctional and blue nevus cells. Non-melanocytic cells do not react with this antibody.

Target Information

Melanoma is a malignant tumor of melanocytes which are found predominantly in skin but also in the bowel and the eye (see uveal melanoma). It is one of the rarer types of skin cancer but causes the majority of skin cancer related deaths.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 95 kDa melanocyte-specific secreted glycoprotein; M-alpha; M-beta; ME20-M; ME20-S; ME20M; melanocyte protein mel 17; Melanocyte protein PMEL; Melanocyte protein Pmel 17; Melanocytes lineage-specific antigen GP100; Melanoma-associated ME20 antigen; melanosomal matrix protein17; P1; P100; P26; Pmel 17; Premelanosome protein; Secreted melanoma-associated ME20 antigen; Silver locus protein homolog; silver, mouse, homolog of

Gene Aliases: D12S53E; gp100; ME20; ME20-M; ME20M; P1; P100; PMEL; PMEL17; SI; SIL; SILV

UniProt ID: (Human) P40967

Entrez Gene ID: (Human) 6490

Molecular Function: cell adhesion molecule membrane-bound signaling molecule signaling molecule