Western blot analysis of PNK in yeast extract (lane 1) and yeast extract + AD-PNK human fusion protein using a PNK polyclonal antibody (Product # PA1-29915) at a dilution of 1:1000 after incubation for 1hr in TBST. The AD fusion is an Activation Domain fusion protein. The band representing PNK is marked by the arrow.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence EPRLGRLYCQFSEG, from C Terminus of the protein sequence according to NP_009185.|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29915 detects PNK from human samples.
PA1-29915 has been successfully used in Western blot applications.
The PA1-29915 immunogen is: Peptide with sequence EPRLGRLYCQFSEG, from C Terminus of the protein sequence according to NP_009185.
The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.