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Invitrogen
PNP Recombinant Rabbit Monoclonal Antibody (001)
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Product Details
MA5-29490
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
001
Immunogen
Recombinant Human PNP protein (Met1-Ser289)
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
This antibody has specificity for Human PNP.
Target Information
The PNP gene encodes for the enzyme purine nucleoside phosphorylase, which plays a vital role in the purine salvage pathway by catalyzing the reversible cleavage of purine nucleosides. This enzyme facilitates the conversion of inosine, guanosine, and deoxyguanosine into free bases and sugars phosphorylated at the C1 position. PNP is located on chromosome 14q13 and is crucial for nucleoside catabolism, impacting energy metabolism and cellular functions across various organisms. PNP deficiency is an autosomal recessive multisystem disorder arising from mutations in the PNP gene, leading to impaired T-cell maturation and function, resulting in recurrent infections, autoimmune manifestations, and neurological abnormalities due to the toxic accumulation of deoxyguanosine triphosphate (dGTP). This deficiency particularly affects immature thymocytes and can lead to lymphoid and neuronal toxicity, underscoring the gene's importance in proper immune system function and cellular health.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein Li 156an; HEL-S-156an; Inosine phosphorylase; Inosine-guanosine phosphorylase; NP; PNP; Purine nucleoside phosphorylase; purine-nucleoside:orthophosphate ribosyltransferase; unnamed protein product
Gene Aliases: NP; PNP; PRO1837; PUNP
UniProt ID: (Human) P00491
Entrez Gene ID: (Human) 4860
nucleoside binding
purine nucleobase binding
catalytic activity
purine-nucleoside phosphorylase activity
protein binding
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring pentosyl groups
phosphate ion binding
identical protein binding
guanosine phosphorylase activity
allantoin metabolic process
nucleobase-containing compound metabolic process
inosine catabolic process
deoxyinosine catabolic process
deoxyadenosine catabolic process
purine ribonucleoside salvage
IMP catabolic process
nicotinamide riboside catabolic process
immune response
nucleoside metabolic process
nucleotide biosynthetic process
response to xenobiotic stimulus
positive regulation of interleukin-2 production
urate biosynthetic process
positive regulation of T cell proliferation
purine-containing compound salvage
dAMP catabolic process
positive regulation of alpha-beta T cell differentiation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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